Arrayit SuperAvidin Microarray Substrate Slides (Box of 25) provide atomically smooth glass substrate slides activated with a covalently bound high-density layer of tetrameric avidin protein, enabling users to print and bind biotinylated DNAs, proteins and other biotin-containing molecules. SuperAvidin substrate slides conform to the standard glass slide format (1 x 25 x 76 mm) and have a corner chamfer to allow unambiguous orientation during use. A time-tested avidin surface for a wide variety of fluorescent DNA and protein microarray applications, provides small printed spots, strong and uniform signals, and low background. Use in conjunction with Arrayit's extensive line of microarray instruments, tools, reagents, buffers and services, 25 slides per box ships on cold packs.
Microarray Slides - SuperAvidin and SuperAvidin Mirror Microarray Substrate Slides Bind Biotinylated DNA and proteins for Life Sciences, Genomics and Proteomics Applications
Arrayit SuperAvidin and SuperAvidin Mirror Microarray Substrate Slides contain a highly uniform tetrameric avidin treatment covalently bound to an atomically smooth glass substrate slide (1 x 25 x 76 mm), affording universal high-capacity biotin-binding surfaces for DNA and protein microarrays and many other life sciences applications that require a biotin binding surface. SuperAvidin Mirror contains a highly reflective backside mirror coating for an additional 2-10X signal enhancement. Arrayit SuperAvidin slides compatible with all standard microarrayers and scanners for optimal customer convenience.
Table of Contents
- Introduction
- Quality Control
- Product Description
- Technical Specifications
- Recommended Equipment and Reagents
- Technical Support
- Scientific Publications
- Ordering Information
- Warranty
Introduction
Congratulations on taking a big step towards improving the affordability, quality and speed of your genomics, biomedical, pharmaceutical and agricultural research. This booklet contains all the information required to take full advantage of Arrayit SuperAvidin Microarray Substrate Slides.
Quality Control
Arrayit takes every measure to assure the quality of our Arrayit SuperAvidin Microarray Substrate Slides. The finest microarray biochip cleanroom research was used to develop this product line. Rigorous quality control monitoring on a part-by-part basis guarantees that each these products conforms to the highest industry standards.
Product Description
Arrayit SuperAvidin Microarray Substrate Slides have covalently-attached tetrameric avidin over the entire 25 x 76 mm surface of the slide for binding biotin-labeled molecules. Arrayit SuperAvidin has a high binding capacity, low background fluorescence for high signal-to-noise ratios and can be used for genomic and proteomic microarrays. The slides conform to the industry standard format (1 x 25 x 76 mm) for compatibility with all standard microarray scanners, and are packaged in slide boxes, vacuum-packed and shipped on cold packs.
Figure 1. Arrayit SuperAvidin Microarray Substrate Slides are saturated with covalently-bound tetrameric avidin protein. Each avidin tetramer on the surface is capable of binding up to four biotinylated molecules. Biotinylated proteins, DNA and other molecules can be bound to this surface to perform a variety of microarray-based binding and hybridization studies.
Short Protocol for Protein (Steps 1-7):
1. Suspend biotinylated protein samples in Protein Printing Buffer (Cat. PPB) at 0.25-1 µg/µl.
2. Print protein samples onto SuperAvidin Microarray Substrate Slides.
3. Block with SuperAvidin Blocking Buffer (Cat. ABB) and then wash and dry the printed microarrays.
4. React the processed microarrays with fluorescent samples.
5. Wash the microarrays to remove un-reacted fluorescent material.
6. Scan the microarray to produce a fluorescent image.
7. Quantitate and model the fluorescent data.
Short Protocol for DNA (Steps 1-7):
1. Suspend biotinylated DNA samples in Arrayit Micro Spotting Solution Plus (Cat. MSP).
2. Print DNA samples onto SuperAvidin Microarray Substrate Slides.
3. Block with SuperAvidin Blocking Buffer (Cat. ABB) and then wash and dry the printed microarrays.
4. Hybridize the processed microarrays with fluorescent probe.
5. Wash the microarrays to remove unreacted fluorescent probe.
6. Scan the microarray to produce a fluorescent image.
7. Quantitate and model the fluorescent data.
Technical Specifications:
SuperAvidin Microarray Substrate Slides are manufactured microarray cleanrooms using atomically smooth polished glass substrate slides followed by activation with a covalent monolayer of a ultra-high purity tetrameric avidin protein. Users will benefit from the following technical features:
- Glass polished to atomic smoothness (±20 angstroms)
- Arrayit offers the only widely-used polished glass surfaces in the microarray industry
- Polishing improves topology and uniformity – keys to precise data
- SuperAvidin Mirror Substrate Slides provide 2-10X signal enhancement
- Homogenous avidin tetrameric protein monolayer provides superior biotin binding
- Avidin molecules bound covalently to the glass substrate slide
- Avidin monolayer contains 1.1 x 10^10 avidin proteins/mm2
- Avidin monolayer covers the entire 25 x 76 mm surface area
- Vastly superior to unpolished optical quality glass from other vendors
- Ultimate avidin surface for high density microarray applications
- Ideal for contact and non-contact printing
- Used by high-volume microarray core facilities
- Manufactured in microarray cleanrooms
- Ultra-low intrinsic fluorescence and low background noise
- Open platform dimensions compatible with all major brands of microarray printers and scanners
- Precise physical dimensions (0.94 ± 0.025 x 25 ± 0.2 mm x 76 ± 0.3 mm)
- Proprietary corner chamfer (1.4 mm) provides unambiguous side and end orientation to simplify printing and processing
- Finished edges enhance user safety
- Optional barcoding streamlines sample tracking
- Excellent refractive index, transmission and hardness specifications
- Vacuum-packaging improves usability
- Offered with or without barcodes
- Custom laser and chrome fiducials available upon request
- High-volume 100,000 piece per month manufacturing capabilities
- Product arrives “ready to use” with no additional processing required
Figure 2. Atomic Force Microscopy (AFM) analysis. Shown are AFM scans of Arrayit SuperClean Microarray Substrate Slides. Data are coded to a rainbow intensity scale, such that red data represents 5.0 nm or 50 angstroms. The substrate slides have an average smoothness of 2.0 nm or 20 angstroms, which corresponds to about 10 silicon dioxide bonds. SuperClean provides the starting point for the SuperAvidin product line.
Figure 3. Correct Substrate Slide Orientation. Shown is a graphic of two Arrayit Microarray Substrate Slides, illustrating correct and incorrect use orientations. In the correct orientation (blue graphic), the chamfer will be located in the upper right corner and samples should be printed on the side facing upward, which is the same side that contains the word “Correct!”. In the incorrect orientation (red graphic), the chamfer will be located in the upper left corner, placing the backside facing upward, which is the side that contains the word “Incorrect!”. Only one side of Arrayit Microarray Substrate Slides is suitable for printing. Please print on the correct side only.
Recommended Equipment and Reagents
NanoPrint™ 2 Protein Microarrayers
SpotBot® 4 Protein Personal Microarrayers
InnoScan® Microarray Scanners
Microarray Hybridization Cassettes
High Throughput Wash Station
Microarray High-Speed Centrifuges
Protein Printing Buffer
BlockIt™ Blocking Buffer
Microarray Air Jet
Microarray Cleanroom Wipes
PCR Purification Kits
Micro-Total RNA Extraction Kit
MiniAmp mRNA Amplification Kit
Indirect Amino Allyl Fluorescent Labeling Kit
Universal Reference mRNA
Green540 and Red640 Reactive Fluorescent Dyes
Hybridization Buffers