Arrayit advanced blood card serum isolation kits allow the isolation of serum from whole blood samples collected and separated on Arrayit Blood Cards. Blood cards separate whole blood into red blood cells, white blood cells and serum components in 5 minutes using unique linear separation chromotography for research, veterinary and in vitro diagnostic applications including genotyping, mRNA expression and protein profiling. Each serum isolation kit contains Serum Elution Buffer (50 ml), 25 spin columns and 25 collection tubes (1.5 ml) for 25 serum isolation reactions using Blood Card membrane discs or whole membranes. Kits arrived pre-mixed and ready to use. Pricing is per 25 serum isolation kit.
Microarray Testing - Arrayit Blood Cards, Private
Label Blood Cards, Blood Card Kits and DNA and Serum Isolation Kits
Arrayit has developed an advanced blood card serum separation technology that
collects and fractionates whole blood into red blood cells, white blood cells
and serum components quickly, easily and affordably. Our unique linear
separation chromatography process collects 5 droplets of whole blood and
separates and dries the whole blood components in 10 minutes. Arrayit Blood
Cards can be used to fractionate, ship, sample and store samples for a variety
of applications in research, veterinary medicine and pathology including
protein-based allergy testing and cancer research. Arrayit Blood Cards can also
be used to isolate DNA and mRNA samples for genotyping and expression profiling
applications. Private labeling is available for large original equipment
manufacturer (OEM) accounts.
Table of Contents
Introduction
Quality Control
Product Description
Product Contents
Whole Blood Contents
Product Protocols
Technical Assistance
Troubleshooting Tips
Recommended Products
Scientific Publications
Ordering Information
Warranty
Introduction
Arrayit has developed an
advanced new blood card serum separation technology for diverse applications in
research, veterinary testing and human in
vitro microarray testing.
Quality Control
Arrayit Blood Cards are
manufactured using the highest level of quality control (QC) and quality
assurance (QA) available. Quality control and quality assurance of each
manufactured lot ensures that our Blood Card performance will meet or exceed
the highest research, veterinary, pathology and microarray testing standards.
Product Description
Arrayit Blood Card serum
separation technology offers an advanced card-based whole blood collection
method that collects, separates and stores whole blood components for research,
veterinary and microarray testing. Users will appreciate the following features
of this product:
Market’s first
high-performance blood card technology
Based on advanced
competitive linear flow chromatography
Separates whole blood
components in less than 5 minutes
Collection, fractionation,
storage and shipping in a single product
Highly purified serum
samples for protein microarrays
Reduces background and
enhances signal strength in antibody assays
Excellent for biomedical
research and pathology applications
Ideal for veterinary
applications including allergy testing
5 drop whole blood
collection capacity
Compatible with both finger
stick and intravenous whole blood samples
6 mm sample disc yields 1-2
µg serum protein
Supports DNA testing
including VIP genotyping assays
Supports mRNA testing
including gene expression profiling assays
Unique patient
identification code ensures proper sample identification
Conventional one-dimensional
barcoding re-confirms sample tracking
Two-dimensional Quick
Response Code (QR Code) permits mobile device web access
Compact 2 x 3.5” (5 x 8.75
cm) “business card” physical dimensions
Extremely lightweight at 1.3
g per card
Customer Reference Card
provides permanent record and easy-to-follow instructions
Compatible with microarrays
and other high-throughput technologies
Easily automated into
96-well sample disc formats
Manufactured and packaged in
a cleanroom environment
Free of biological and
chemical contaminants
Ultra-thin proprietary
membrane speeds fractionation and improves sample integrity
Suitable for parentage and
forensic testing applications
Reduces sample shipping
costs and conserves laboratory space
10-year DNA and protein
stability
Figure 1. Arrayit Private Label
Blood Cards (Cat. ABCP) and Blood Card Kits with logo, user instructions,
company address and custom QR codes are available for large OEM customers.
Arrayit Blood Card technology is for research use only.
Blood Card Kit Contents
Blood Card, 1 each
Customer Reference Card, 1
each
Fingerstick lancets, 2 each
Silver anti-static shipping
envelope, 1 each
Whole Blood Contents
Arrayit Blood Cards collect
5 droplets of human blood, which contains the following cellular and molecular
components:
100 µl of whole human blood
4.8 x 10-8 red
blood cells
7.3 x 10-5 white
blood cells
40 µl of serum
2.9 mg total serum protein
250 µg total serum protein
(6 mm disc)
2.5 µg white blood cell
total RNA (6 mm disc)
0.5 µg total genomic DNA (6
mm disc)
Serum Isolation Kit Contents (25 samples)
Spin columns, 25 each
Microfuge tubes (1.5 ml), 25
each
Serum elution buffer (1X),
50 ml of 1X
DNA Isolation Kit Contents (25 samples)
Spin columns, 25 each
Microfuge tubes (1.5 ml), 75
each
Microfuge wash tubes (1.5
ml), 25 each
DNA Buffer A (1X), 15 ml
DNA Buffer B (1X), 15 ml
DNA Buffer C (1.33X), 15 ml
DNA Buffer D (1X), 15 ml
DNA Buffer E (1X), 15 ml
Figure 2. Arrayit Blood Card Kits
(Cat. ABCK) include a Customer Reference Card as a permanent record of the
sample. The Reference Card contains a Patient ID number that matches the Blood
Card, a traditional barcode with serial number and a Quick Reference Code (QR
Code) two-dimensional barcode for accessing the Blood Card webpage using smart
phones, tablets and other mobile devices. The Customer Reference Card also
includes space for a hand-written patient name and sampling date. The Reference
Card has business card dimensions of 2 x 3.5” (5 x 8.9 cm) for convenient
storage and referencing. The back of the card contains instructions for blood
sampling (see Figure 3).
Figure 3. Arrayit Blood Card Kits
(Cat. ABCK) include Blood Card Instructions as a 9-step blood sampling
procedure on the back of the Customer Reference Card (see Figure 2 for front
view). The Reference Card has business card dimensions of 2 x 3.5” (5 x 8.9 cm)
for convenient storage and referencing. The front of the Reference Card
contains a Patient ID number that matches the Blood Card, a traditional barcode
with serial number, a Quick Reference Code (QR Code) two-dimensional barcode
for accessing the Blood Card webpage using smart phones, tablets and other
mobile devices, and a space for a hand-written patient name and sampling date
(see Figure 2).
Figure 4. Arrayit Blood Card Kits
(Cat. ABCK) contain a Blood Card, Customer Reference Card, two fingerstick
lancets, and a silver anti-static envelop for shipping. Cut the plastic kit
pouch with a scissors to remove the kit components and follow instructions
carefully before, during and after use.
Figure 5. Arrayit Blood Card
fingerstick lancet. Blood sampling is achieved by placing the middle finger on
your left hand directly on the red, spring-loaded sampling platform as shown
and pressing down firmly. Follow instructions carefully before, during and
after use.
Figure 6. Arrayit Blood Card shown
10 minutes after placing 5 large drops of whole human blood inside the red box.
The red blood cell components remain on the left portion of the linear transfer
membrane and the serum (yellowish fraction) partitions to the right. Arrayit
Blood Cards can be used for DNA, mRNA and protein analysis by taking sample
discs of these respective fractions.
Blood Sampling (Short Protocol)
1. Wash your hands with soap
and hot water for 1 minute and dry your hands with a lint-free wipe.
2. Open the plastic product
envelop and place the kit components on the bench top.
3. Remove the plastic safety
cap from the lancet.
4. Place the lancet on the
bench top with the red sampling platform facing upward.
5. Place your left middle
fingertip on the lancet sampling platform.
6. Press down firmly on the
lancet.
7. Place 5 large drops of
whole blood inside the red box on the Blood Card.
8. Allow the blood to
separate on the Blood Card for 5 minutes.
9. Allow the Blood Card to
dry for 5 minutes.
10. Place the Blood Card in
the silver anti-static shipping envelop for storage and shipping.
Blood Sampling (Complete Protocol)
1. Wash your hands with soap
and hot water for 1 minute and dry your hands with a lint-free wipe. This step
is important because it removes bacteria and other contaminants from your hands
prior to sampling. The use of hot water also improves blood circulation in your
finger tips, which improves the efficiency of the sampling procedure. Make sure
to wash for at least 1 minute as shorter wash periods will reduce blood flow.
Please avoid topical cleaning with alcohol swabs and other solvents as this
will dehydrate the sampling area and reduce blood flow. Make sure to dry your
hands completely with a lint-free laboratory wipe to ensure dry finger tips
prior to blood sampling.
2. Open the plastic product
envelop and place the kit components on the bench top. Use a scissors or other
cutting implement to carefully cut the top of the plastic envelop and then
remove the Blood Card, Customer Reference Card, two lancets and the silver
anti-static shipping envelope. The kit components (see Figure 4) should be
placed on a smooth, clean and level surface such as a laboratory bench or
clinical table.
3. Remove the plastic safety
cap from the lancet. The pink lancets contain a clear plastic safety cap on the
top of the lancet to protect the lancet from premature use. Remove the plastic
cap carefully immediately prior to use.
4. Place the lancet on the
laboratory bench with the red sampling platform facing upward. The lancet
contains a red, spring-loaded sample platform. Make sure the sampling platform
is facing upwards prior to sampling.
5. Place your left middle
fingertip on the lancet sampling platform. Place the tip of your middle finger
softly in the center of the sampling platform with your finger tip aligned
directly in the center of the 3 mm orifice located in the center of the
platform (see Figure 5). Because of the proximity to your heart, blood pressure
and blood flow in the left hand is slightly greater than in your right hand,
and the left middle finger is chosen for this reason.
6. Press down firmly on the
lancet. Press down firmly on the lancet platform to initiate the sampling
process. The lancet uses a spring-loaded stainless steel sampling device
approximately 350 µm in diameter to create a nearly invisible sampling site on
your left middle finger tip. The sampling process is fast and nearly
undetectable.
7. Place 5 large drops of
whole blood inside the red box on the Blood Card. For best results, make sure
you are standing up for this step to increase blood pressure and blood flow.
Use your non-sampling hand to squeeze your finger tip firmly and create five
blood droplets. Place the droplets directly in the center of the red box of the
Blood Card, making light contact between the droplet and the Blood Card
membrane. Avoid touching the Blood Card membrane directly with your fingertip
as this may interfere with the separation process. A properly lanced finger tip
should produce 5 large drops or 100 µl of whole blood in less than one minute.
If blood flow stops, it may be necessary to use the second lancet provided with
the kit to obtain 5 large blood droplets.
8. Allow the blood to
separate on the Blood Card for 5 minutes. After placing 5 large blood droplets
in the center of the red box, allow 5 minutes for the blood components to
separate on the Blood Card. The Blood Card should be placed level and flat on
the laboratory bench during the separation process to permit even flow of the
blood components. The blood components should separate from left to right on
the card such that the red cell components remain to the left side of the
separation membrane and the yellowish serum components migrate rightward during
the 5 minute process. Five large blood droplets should produce a fractionation
result similar to Figure 6. Customers using whole blood obtained by intravenous
blood draws should place 5 drops or 100 µl of blood inside the red box.
9. Allow the Blood Card to
dry for 5 minutes. Following the 5 minute fractionation process, incubate the
Blood Card on the laboratory bench for an additional 5 minutes to allow drying
of the fractionated whole blood components. The drying process enhances the
stability of the blood components for sampling and storage and ensures that the
components will remain firmly adhered to the Blood Card during shipping. Avoid
making direct contact with the separated blood components at all times during
the sampling, fractionation and drying steps as this can contaminate the samples
and complicate downstream analysis.
10. Place the Blood Card in
the silver anti-static shipping envelope for storage and mailing. Open the
zip-lock located at the top of the silver anti-static shipping envelope and
place the dried Blood Card into the envelope. Re-seal the zip-lock located at
the top of the silver envelope and mail to the proper laboratory for testing.
Alternatively, the Blood Card in the sealed silver anti-static envelope can be
stored in a cool dark drawer or storage cabinet for future testing. Make
certain to retain your Customer Reference Card as a permanent record of your
sample, making sure to write your name and the sampling date on the card for
future reference. The patient identification number located on your reference
card is identical to the patient identification number on the Blood Card.
Figure 7. Arrayit Blood Card sample
disc isolated 24 hours after whole blood collection. A 6 mm round disc
containing fractionated serum was obtained using the Arrayit Blood Card Sample
Disc Punch (Cat. ASDP). Serum discs of this diameter contain approximately 250
µg of total serum protein containing peptides, antigens, antibodies and other
analytes of scientific, research, veterinary and pathology interest.
Figure 8. Arrayit Blood Card Sample
Disc Punch (Cat. ASDP) enables precision manual generation of 6 mm sample discs
for DNA, mRNA and serum samples. The disc punch is manufactured using injection
molded plastic for resistance to light solvents. Spring loading allows easy
manual operation. Sample discs are automatically released from the disc punch
to allow easy transfer into microfuge tubes and microplates.
DNA Isolation (Short Protocol)
Note: Please wear nitrile
gloves and safety glasses at all times during this procedure.
1. Obtain a 6 mm sample disc
from the blood card.
2. Place the sample disc in
a 1.5 ml microfuge tube.
3. Add 200 µl DNA Buffer A
and 100 µl DNA Buffer B to the microfuge tube.
4. Vortex the microfuge tube
vigorously for 20 sec and incubate 10 min.
5. Centrifuge the microfuge
tube at 14,000 x g for 2 min.
6. Transfer the 100 µl
aqueous (top) fraction to a fresh 1.5 ml microfuge tube.
7. Re-extract the sample
disc two times with 50 µl DNA Buffer B.
8. Combine the three aqueous
(top) fractions to create a 200 µl volume of DNA sample.
9. Add 200 µl DNA Buffer A
to the 200 µl aqueous DNA sample.
10. Vortex the microfuge
tube vigorously for 20 sec.
11. Centrifuge the microfuge
tube at 18,000 x g for 2 min.
12. Transfer the 200 µl
aqueous (top) fraction to a fresh 1.5 ml microfuge tube.
13. Add 600 µl of DNA Buffer
C and vortex for 10 sec.
14. Transfer the 800 µl DNA
sample to a spin column seated in a microfuge wash tube.
15. Centrifuge the spin
column assembly at 1,000 rpm for 10 sec and discard the eluent.
16. Add 125 µl of DNA Buffer
D to the spin column.
17. Centrifuge the spin
column assembly at 1,000 rpm for 10 sec and discard the eluent.
18. Repeat wash steps 16 and
17 twice more.
19. Centrifuge the spin
column assembly at 1,000 rpm for 60 sec to dry the spin column.
20. Add 50 µl of DNA Buffer
E directly onto the spin column membrane.
21. Incubate the spin column
for 5 min to resolubilize the DNA.
22. Centrifuge the spin
column assembly at 1,000 rpm for 60 sec to elute the DNA.
23. Dry the DNA sample by
vacuum centrifugation for downstream use.
DNA Isolation (Complete Protocol)
Note: Please wear nitrile
gloves and safety glasses at all times during this procedure.
1. Obtain a 6 mm sample disc
from the blood card. Use an Arrayit Sample Disc Punch to obtain a 6 mm round
sample disc from the cellular “red” portion of the blood card. The sample disc
will include white blood cells that contain nuclear and mitochondrial DNA. The
plunger of the Disc Punch should be cleaned with distilled water and 100%
ethanol after each use to prevent cross-contamination.
2. Place the disc in a 1.5
ml microfuge tube. Use clean forceps to carefully transfer the sample disc to a
1.5 ml microfuge tube.
3. Add 200 µl DNA Buffer A
and 100 µl DNA Buffer B to the microfuge tube. Pipette 200 µl of DNA Buffer A
and 100 µl of DNA Buffer B into the 1.5 ml microfuge tube. These buffers
disrupt blood cells, inactivate cellular DNases that can degrade DNA and
solubilize the DNA into the aqueous phase.
4. Vortex the microfuge tube
vigorously for 20 sec and incubate 10 min. Close the 1.5 ml microfuge tube cap
and vortex the tube vigorously for 20 seconds to disrupt the cells and elute
the DNA from the blood card membrane. The 10 minute incubation at room
temperature will ensure complete cellular disruption and efficient DNA
recovery.
5. Centrifuge the microfuge
tube at 14,000 rpm for 2 min. Transfer the 1.5 ml microfuge tube to a
microcentrifuge and spin the tube at the full speed of 14,000 revolutions per
minute (rpm) or 18,000 x g for 2 minutes to separate the phases. The blood card
sample disc and insoluble material partitions in the bottom layer and the DNA
partitions in the soluble aqueous (top) layer. The bottom layer should appear
dark red and the top layer should appear clear to lightly beige in color.
6. Transfer the 100 µl
aqueous (top) fraction to a fresh 1.5 ml microfuge tube. Use a 200 µl pipette
tip with a barrier plug to transfer the 100 µl top layer containing the DNA to
a fresh 1.5 ml microfuge tube. Pipette the top layer slowly and carefully to
avoid transferring the bottom layer or interface.
7. Re-extract the sample
disc two times with 50 µl DNA Buffer B. After transferring the 100 µl top layer
containing the DNA, add 50 µl of fresh DNA Buffer B to the bottom layer in the
1.5 ml microfuge tube and vortex the tube vigorously for 20 seconds to disrupt
additional cells and solubilize additional DNA. Centrifuge the 1.5 ml microfuge
tube at 14,000 rpm for 2 minutes to separate the phases, and transfer the 50 µl
top layer to the microfuge tube containing the 100 µl DNA sample. Repeat this
process once more and transfer the 50 µl top layer to the microfuge tube
containing the 150 µl DNA sample.
8. Combine the three aqueous
(top) fractions to create a 200 µl volume of DNA sample. After the three
extraction cycles, the 1.5 ml microfuge tube should contain a total volume of
200 µl containing the DNA sample. The DNA sample should appear clear to lightly
beige in color.
9. Add 200 µl DNA Buffer A
to the 200 µl aqueous DNA sample. To the 1.5 ml microfuge tube containing the
200 µl DNA sample, add 200 µl of DNA Buffer A using a 1 ml pipette tip.
10. Vortex the microfuge
tube vigorously for 20 sec. Obtain the 1.5 ml microfuge tube containing 200 µl
of DNA sample and 200 µl of DNA Buffer A and vortex the tube vigorously for 20
seconds to mix the phases and remove any remaining insoluble material from the
DNA sample.
11. Centrifuge the microfuge
tube at 18,000 x g for 2 min. Transfer the 1.5 ml microfuge tube containing 200
µl of DNA sample and 200 µl of DNA Buffer A to a microcentrifuge and centrifuge
the tube at 14,000 rpm for 2 minutes to separate the phases.
12. Transfer the 200 µl
aqueous (top) fraction to a fresh 1.5 ml microfuge tube. After the 2 minute
centrifugation step, remove the 1.5 ml tube from the microcentrifuge, open the
cap and remove the 200 µl DNA sample using a 200 µl tip fitted with barrier
plug. Pipette the top layer slowly and carefully to avoid transferring the
bottom layer or interface. Transfer the 200 µl aqueous DNA sample to a fresh
1.5 ml microfuge tube.
13. Add 600 µl of DNA Buffer
C and vortex for 10 sec. To the 200 µl aqueous DNA sample, add 600 µl of DNA
Buffer C using a 1.0 ml pipette tip, close the cap of the 1.5 ml microfuge tube
and vortex vigorously for 10 seconds to mix the DNA sample and DNA Buffer C.
The total sample volume at this step is 800 µl.
14. Transfer the 800 µl DNA
sample to a spin column seated in a microfuge wash tube. Place a spin column
into a 2 ml microfuge wash tube and add the 800 µl DNA sample directly onto the
spin column membrane making certain to avoid contact between the membrane and
the 1.0 ml pipette tip.
15. Centrifuge the spin
column assembly at 1,000 rpm for 10 sec and discard the eluent. Centrifuge the
spin column assembly containing the 800 µl DNA sample at 1,000 rpm or 1,250 x g
for 10 seconds to trap the DNA molecules on the spin column membrane. The
buffer components and contaminants will pass through the spin column membrane
into the 1.5 ml microfuge wash tube. After the 10 second centrifugation step,
remove the spin column assembly from the microcentrifuge and discard the 800 µl
eluent in the 1.5 ml microfuge wash tube containing the buffer components and
contaminants. At this step, the DNA molecules adhere to the spin column membrane.
16. Add 125 µl of DNA Buffer
D to the spin column. After decanting the 800 µl flow-through, place the spin
column containing the DNA molecules into the 1.5 ml microfuge wash tube and add
125 µl of DNA Buffer D using a 200 µl pipette tip. This step removes residual
contaminants from the spin column membrane.
17. Centrifuge the spin
column assembly at 1,000 rpm for 10 sec and discard the eluent. After adding
125 µl of Wash Buffer D to the spin column, centrifuge the spin column assembly
at 1,000 rpm for 10 seconds to wash the spin column membrane and discard the
flow through. Place the spin column back into the 1.5 ml microfuge wash tube
and place the spin column assembly in the microcentrifuge.
18. Repeat wash steps 16 and
17 twice more. After the first 125 µl wash step with Wash Buffer D, repeat this
step (steps 16 and 17) two more times to thoroughly wash the spin column
membrane. The three wash steps ensure a highly purified DNA preparation upon
elution.
19. Centrifuge the spin
column assembly at 1,000 rpm for 60 sec to dry the spin column. After the three
wash steps, insert the spin column assembly into the microcentrifuge and
centrifuge at 1,000 rpm for 60 seconds to dry the spin column completely. This
step ensures efficient elution of the DNA sample from the spin column membrane.
20. Add 50 µl of DNA Buffer
E directly onto the spin column membrane. After the 60 second centrifugation
step, remove the spin column assembly from the microcentrifuge and discard the
1.5 ml microfuge wash tube. Place the spin column containing the highly
purified DNA molecules into a fresh 1.5 ml microfuge tube and add 50 µl of DNA
Buffer E directly onto the spin column membrane using a 200 µl pipette tip.
21. Incubate the spin column
for 5 min to resolubilize the DNA. The 50 µl volume of DNA Buffer E should be
allowed to incubate for 5 minutes on the spin column membrane to resolubilize
the DNA molecules. Make sure that DNA Buffer E covers the entire membrane
during this step.
22. Centrifuge the spin
column assembly at 1,000 rpm for 60 sec to elute the DNA. After the 5 minute
incubation step, centrifuge the spin column assembly at 1,000 rpm for 60
seconds to elute the DNA sample from the membrane. The 50 µl flow through will
collect in the bottom of the 1.5 ml microfuge tube. Discard the spin column and
retain the 1.5 ml microfuge tube that contains the purified DNA sample.
23. Dry the DNA sample by
vacuum centrifugation for downstream use. After the DNA elution step, place the
1.5 ml microfuge tube containing the 50 µl purified DNA sample in a vacuum
centrifuge (e.g. SpeedVac®) and centrifuge the sample for 30 minutes to dry the
DNA pellet completely. A 6 mm sample disc should provide approximately 0.5 µg
of total genomic DNA.
Serum Isolation (Short Protocol)
Note: Please wear nitrile
gloves and safety glasses at all times during this procedure.
1. Excise 6 mm disc from
serum portion of blood card.
2. Wet 6 mm disc with 10 µl
of 1X Serum Elution Buffer.
3. Place wetted disc in
Serum Spin Column assembly.
4. Re-hydrate disc for 30
min at room temperature.
5. Spin assembly for 1 min
at 14,000 x g to elute serum.
6. Add 10 µl of 1X Serum
Elution Buffer to disc.
7. Spin assembly for 1 min
at 14,000 x g to elute residual serum.
8. Mix and react 20 µl serum
sample with protein microarray.
Serum Isolation (Complete Protocol)
Note: Please wear nitrile
gloves and safety glasses at all times during this procedure.
1. Excise 6 mm disc from
serum portion of blood card. Obtain Blood Card (Cat. ABC) containing a chromatographed
5-drop whole blood sample into the red portion (left) and serum protein
(right). Use an Arrayit Blood Card Sample Disc Punch (Cat. ASDP) to excise a 6
millimeter (6 mm) disc from the serum portion of the card. The 6 mm will
contains antibodies, antigens and other serum proteins of interest.
2. Wet 6 mm disc with 10 µl
of 1X Serum Elution Buffer. Using a 20 µl pipette fitted with a 20 µl barrier
tip, wet the 6 mm serum disc with 10 µl of 1X Serum Elution Buffer. The filter
disc should be held and handled using fine-tipped forceps.
3. Place wetted disc in
Serum Spin Column assembly. Place a Serum Spin Column inside a 1.5 ml Serum
Collection Tube to form a Serum Spin Column assembly. Place the 6 mm filter
disc wetted with 10 µl of 1X Serum Elution Buffer inside the Serum Spin Column
portion of the Serum Spin Column assembly.
4. Re-hydrate disc for 30
min at room temperature. The wetted 6 mm serum disc inside the Serum Spin
Column assembly should be incubated for 30 min at room temperature to allow
re-hydration of the serum proteins. The assembly should be held in a microfuge
rack or microfuge rotor in an upright position during 30 min incubation.
5. Spin assembly for 1 min
at 14,000 x g to elute serum. The Serum Spin Column assembly containing the 6
mm disc wetted with 10 µl of 1X Serum Elution Buffer should be centrifuged for
1 min at 14,000 x g to elute the serum proteins off the disc and into the 1.5
ml Serum Collection Tube. The volume of the eluted and collected sample should
be approximately 10 µl.
6. Add 10 µl of 1X Serum
Elution Buffer to disc. Following the first elution, a small percentage of
serum proteins remain bound to the 6 mm disc. To remove the remaining bound
proteins, add a second 10 µl aliquot of 1X Serum Elution Buffer to the 6 mm
disc. The two 10 µl aliquots of 1X Serum Elution Buffer will afford a 20 µl
final sample volume. Certain protein microarray assays may require an assay
volume greater than 20 µl. In this case, simply increase the volume of the
second elution to a volume that is 10 µl less than the assay volume. To obtain
a 100 µl final assay volume, for example, the first and second elution volumes
should be 10 µl and 90 µl, respectively.
7. Spin assembly for 1 min
at 14,000 x g to elute residual serum. After the second 10 µl volume of 1X
Serum Elution Buffer is added to the 6 mm serum disc, spin the Serum Spin
Column assembly for 1 min at 14,000 x g in a microfuge to elute the serum
proteins off the disc and into the 1.5 ml Serum Collection Tube. If two 10 µl volumes
of 1X Serum Elution Buffer are used for the elutions, the final volume of
eluted serum proteins should be approximately 20 µl. The serum protein elution
volume will be higher for assays that use a larger second elution volume.
8. Mix and react 20 µl serum
sample with protein microarray. The 1.5 ml Serum Collection Tube will contain
the eluted serum sample from the two elutions. For the standard elution
protocol that uses 2 x 10 µl of 1X Serum Elution Buffer, the eluted sample will
be approximately 20 µl. Mix the eluted sample gently for 5 sec using a rotary
vortex to ensure that the sample is completely homogeneous before applying the
eluted serum sample to the protein microarray.
Technical Assistance
Please contact us if you
have any comments, suggestions, or if you need technical assistance. We can be
reached by electronic mail arrayit@arrayit.com from
Monday-Friday 8:00 AM-8 PM PST. Please remember that we want to hear about
your successes!
Troubleshooting Tips
The lancet is not creating
sufficient blood flow - make sure to press down firmly on the lancet sampling
platform during the sampling process
Blood flow stops before 5
droplets are collected - make sure to wash your hands with hot soapy water
prior to sampling and stand up during the collection process
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Scientific Publications
Click here and here for
Arrayit blood card scientific publications.
Ordering Information
Product
|
Description
|
Catalog
ID
|
Arrayit Blood Cards
|
Arrayit Blood Cards enable
collection, fractionation, sampling, shipping and storage with a single
device. Separate whole blood into red blood cells, white blood cells and
serum components in 5 minutes using unique linear separation chromatography
for research, veterinary and pathology applications including genotyping,
mRNA expression and protein profiling. Each card contains a traditional
barcode with serial numeric, two-dimensional QR Code for mobile device access
and patient ID for unique sample identification. Research use only. Includes
blood card and anti-static shipping envelop, 1 card per kit.
|
ABC
|
Arrayit Private Label
Blood Cards
|
Arrayit Private Label
Blood Cards enable collection, fractionation, sampling, shipping and storage
with a single device. Separate whole blood into red blood cells, white blood
cells and serum components in 5 minutes using unique linear separation
chromatography for research, veterinary and pathology applications including
genotyping, mRNA expression and protein profiling. Each card contains a
traditional barcode with serial numeric, custom two-dimensional QR Code for
mobile device access and patient ID for unique sample identification. This
product is sold for research use only. Includes private label blood card and
anti-static shipping envelop, 1 private label card per kit.
|
ABCP
|
Arrayit Blood Card
Sampling Kits
|
Arrayit Blood Card Kits
enable collection, fractionation, sampling, shipping and storage with a
single blood kit. Separate whole blood into red blood cells, white blood
cells and serum components in 5 minutes using unique linear separation
chromatography for research, veterinary and pathology applications including
genotyping, mRNA expression and protein profiling. Each kit contains a Blood
Card, Customer Reference Card, two lancets and an anti-static shipping
envelope. Kit components are labeled with traditional barcodes containing a
serial numeric, two-dimensional QR Codes for mobile device access and patient
IDs for unique sample identification. This product is sold for research use
only. Pricing is for 1 kit.
|
ABCK
|
Arrayit Blood Card Serum
Isolation Kit
|
Arrayit Blood Card Serum
Isolation Kit enables the isolation and purification of serum and plasma from
whole blood samples collected on blood cards. Kit contents include Spin
Columns (25 each), 1.5 ml Collection Tubes (25 each), 1X Serum Elution Buffer
(50 ml), wear nitrile gloves and safety glasses at all times during use,
store at room temperature. This product is sold for research use only.
Pricing is per kit for 25 samples.
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ABCS
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Arrayit Blood Card DNA
Isolation Kit
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Arrayit Blood Card DNA
Isolation Kit enables the isolation and purification of total genomic DNA
from whole blood samples collected on blood cards. Kit contents include spin
columns (25 each), 1.5 ml microfuge tubes (25 each), 1.5 ml microfuge wash
tubes (25 each), DNA Buffer A (15 ml), DNA Buffer B (15 ml), DNA Buffer C (15
ml), DNA Buffer D (15 ml), and DNA Buffer E (15 ml), wear nitrile gloves and
safety glasses at all times during use, store at room temperature. This
product is sold for research use only. Pricing is per kit for 25 samples.
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DNA
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Arrayit Blood Card Sample
Disc Punch
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Arrayit Blood Card Sample
Disc Punch for precision manual generation of 6 mm sample discs for DNA, mRNA
and serum samples, injection molded plastic, durable laboratory tool, resistant
to light solvents, spring loaded for easy manual operation, automatically
releases sample discs for easy disc transfer into microfuge tubes and
microplates. Pricing is for 1 disc punch.
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ASDP
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Warranty
Arrayit life sciences
products are sold for research purposes only. Arrayit brand products have been
scientifically developed and are sold for research purposes. Extreme care and
exact attention should be practiced in the use of the materials described
herein. All Arrayit brand products are subject to extensive quality control and
are guaranteed to perform as described when used properly. Any performance
issues should be reported to Arrayit immediately. Arrayit’s liability is
limited to the replacement of the product, or a full refund. Any misuse of this
product is the full responsibility of the user, and Arrayit makes no warranty
or guarantee under such circumstances. Pricing may vary up to 30% due to costs
associated with distribution, import taxes, duties, customs clearance and
shipping.