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Primary Cilia Pathways™ Focused Human Genome Microarrays


Price: $1,036.00
Item Number: PRPM
Arrayit Pathways™ Focused Human Genome Microarrays enable targeted gene expression analysis of every major cellular pathway in the human body. The content is derived from our high performance H25K human genome microarrays, which contain a complete set of 25,509 fully annotated human genes representing every gene in the human genome. Pathways™ Microarrays offer the highest sensitivity and specificity of any product on the market, empowered by long-mer oligonucleotides, proprietary attachment and surface chemistry, and a unique one gene-one spot™ design. Pathways™ Microarrays allow users to rapidly and economically explore adipogenesis, angiogenesis, apoptosis, cancer drug targets, cardiotoxicity, cell cycle, cell motility, cytokine production, DNA repair, glucose metabolism, growth factors, hypertension, JAK/STAT, mitochondria, neurotoxicity, protein phoshatases, stem cells, transcription factors, WNT signalling pathways and many others. Arrayit offers a complete line of instruments, tools, kits, reagents and software to fully support Pathways™ Microarrays experimentation. P13 - AKT Signaling Pathways™ Focused Human Genome Microarrays contain 89 long-mer capture agents for 89 genes involved in the human P13 - AKT signaling pathway, printed on 25 x 76 mm glass substrate slides, 10 microarrays per kit.

Reagents - DNA Microarrays - Pathways™ Focused Human Genome Microarrays

pathway-microarray
Arrayit Pathways™ Focused Human Genome Microarrays enable targeted gene expression analysis of every major cellular pathway in the human body. The content is derived from our high performance H25K human genome microarrays, which contain a complete set of 25,509 fully annotated human genes representing every gene in the human genome. Pathways™ Microarrays offer the highest sensitivity and specificity of any product on the market, empowered by genes, proprietary attachment and surface chemistry, and a unique one gene-one spot™ design. Pathways™ Microarrays allow users to rapidly and economically explore adipogenesis, angiogenesis, apoptosis, cancer drug targets, cardiotoxicity, cell cycle, cell motility, cytokine production, DNA repair, glucose metabolism, growth factors, hypertension, JAK/STAT, mitochondria, neurotoxicity, protein phoshatases, stem cells, transcription factors, WNT signalling pathways and many others. Arrayit offers a complete line of instruments, tools, kits, reagents and software to fully support Pathways™ Microarrays experimentation.

Contents

  • Introduction
  • Quality Control
  • Product Description
  • Kit Contents
  • Technical Notes
  • Short Protocols
  • Complete Protocols
  • Technical Assistance
  • Troubleshooting Tips
  • Recommended Equipment
  • Ordering Information
  • Storage Conditions
  • Warranty

Introduction
The use of ArrayIt® brand products will improve the precision, speed and affordability of your microarray research in genomics, proteomics, biomedicine, pharmaceuticals and diagnostics. This handbook contains all the information required to take full advantage of Arrayit's Pathway Focused DNA Microarrays.

Quality Control
Arrayit uses the highest quality control (QC) and quality assurance (QA) measures to ensure the quality of our ArrayIt® Pathways Human DNA Microarrays. The finest science and engineering was used to develop this product. The use of advanced bioinformatics, ultra-pure oligonucleotides, superior surface chemistry and advanced microarray cleanroom manufacturing facilities guarantees that Pathways™ Microarrays outperform the highest industry standards.

Product Description
Arrayit Pathways™ Focused Microarrays represent the most advanced human DNA microarrays in the industry. These microarrays are designed using the complete, fully annotated human genome sequence using sophisticated one spot-one gene™ informatics, useding highest gene content available. These focused microarrays are manufactured in advanced cleanrooms using our patented contact printing technology (U.S. 6,101,946), with the strictest QC and QA procedures. All our microarrays offer quality and performance that cannot be obtained from any other microarray, PCR or next generation sequencing approach. These microarrays are compatible with all slide based microarray scanners.

Users will appreciate the following features of the Pathways Focused DNA Microarrays:

  • Designed from the complete, fully annotated human genome sequence
  • Annotated across GenBank, UniGene, GoldenPath, RefSeq and AceView
  • Made using long oligonucleotide elements
  • Each gene target printed in triplicate to enhance statistical analysis
  • Novel one spot-one gene™ design streamlines data analysis
  • Explore the function human genes in your targeted area of interest (see list below)
  • Devoid of pseudogenes, and redundant and chimeric sequences
  • Control sequences add precision and reliability
  • Contains widely used Ambion and Stratagene controls
  • Detect many human mRNAs in a single hybridization reaction
  • Manufactured using Arrayit’s patented printing technology (U.S. 6,101,946)
  • Oligonucleotides made “off-line” exceed 90% purity levels
  • Printed on atomically smooth glass substrates for improved precision
  • Proprietary series 3 Arrayit glass offers world’s lowest intrinsic fluorescence
  • Compatible with an installed base of >10,000 slide scanners
  • Allow validation of Affymetrix, Agilent, ABI, GE, Qiagen PCR arrays and other vendors
  • 10 µl reaction volume accelerates kinetics and minimizes sample consumption
  • Format compatible with all standard data quantification and mining packages
  • Compatible with dozens of DNA and RNA amplification and labeling schemes
  • Utilize fluorescent, colorimetric and chemilluminscent detection
  • One, two, three and four color detection possible
  • Detect limit: 1 mRNA per 1,000,000
  • Dynamic range: >1,000,000-fold
  • Chip-to-chip CVs: ±20%
  • Corner chamfer provides unambiguous spatial orientation
  • Barcode assists in data tracking
  • Sophisticated anti-static packaging improves usability
  • Produced in our automated manufacturing facilities
  • Microarrays are shipped ready for hybridization.

Kit Contents
Each Pathways kit contains the following components:

  • 10 x Pathways™ Focused Human Gene Expression Microarrays
  • 10 x Cover Slips (18 x 18 mm)
  • 1 x Pathway Gene List
  • 1 x GAL File Quantification Spot Map

DNA-microarrays
Figure 2.
Pathways Focused DNA Microarays use the same packaging as our H25K Human Genome Microarray. Each kit manufactured and packaged in cleanrooms and heat-sealed in anti-static packaging to ensure optimal performance and indefinate shelf life if stored clean and dry.

Gene Content
Arrayit Pathways™ Microarrays utilize subsets of the gene content present on the Arrayit H25K Human Genome Microarray, which includes 26,304 features or “spots” representing 25,509 fully annotated human genes and 795 control sequences from mouse, plant and other sources. Pathways™ Microarrays oligonucleotides contain sense strand information, and therefore nucleic acid probe mixtures reacted with these microarrays must contain anti-sense molecules to hybridize to the sense oligonucleotides present on the chip. The long oligonucleotide sequences present in each feature are annotated across the AceView, Entrez, RefSeq, UniGene, KnownID, and GenBank databases. Each oligonucleotide also includes a unique MicroarrayID, which identifies the sequence on microarary and allows customers to purchase the sequence by specifying the MicroarrayID. Gene descriptions are based on the best descriptions available from GenBank and AceView. Gene lists for Pathways™ Mcroarray are provided by electronic mail at arrayit@arrayit.com.

Short Protocol (Steps 1-7)
1. Prepare a labeled probe from a biological sample.
3. React the probe sample with a Pathways™ Microarray to allow molecular binding.
4. Wash the Pathways™ Microarray to remove unbound probe molecules.
5. Detect Pathways™ Microarray signals by scanning or imaging.
6. Quantify the Pathways™ Microarray signals.
7. Perform data analysis.

Complete Protocol (Steps 1-7)
1. Prepare a labeled probe from a biological sample. Pathways™ Microarrays contain specific sets of long oligonucleotides representing genes in a specific Pathways in the human genome. These microarrays can be used to query a single probe mixture, or multiple probes prepared using multiple fluorescent labels in multi-color DNA and RNA hybridization. For gene expression applications, each oligonucleotide designed to be complimentary to the 3’ end of a gene and is optimized to hybridize to as many transcripts as possible in cases where genes express multiple mRNAs. Each spot will detect a majority of transcripts for every gene on the microarray single reaction. There are three fundamental principles that should be used for sample preparation: (1) Sample integrity must be high. DNA, mRNA and protein molecules must be full length and free of contaminating enzymes that can produce nucleic acid and protein degradation. (2) Probe labeling must be efficient. Probes should be checked prior to reactions with to ensure that the probe mixtures are highly fluorescent. The most common cause of poor performance is poor sample/probe preparation. (3) Probe mixtures must be pure. Prior to hybridization, probe mixtures should be purified to remove non-incorporated dyes and other contaminants that can increase background noise. This step is well worth the small investment in time and money, as it will greatly increase performance. Direct and indirect fluorescent and non-fluorescent labeling techniques have been described extensively in the literature and a host of commercial labeling kits are available from many different vendors. Arrayit offers an extensive line of purification, amplification and labeling kits for DNA, mRNA and protein labeling applications. For gene expression applications, Arrayit’s Micro-Total RNA Extraction Kit, MiniAmp mRNA Amplification Kit, Indirect Amino Allyl Fluorescent Labeling Kit, and Universal Reference mRNA are highly recommended for maximum signal strength.

2. React the probe sample with microarray to allow molecular binding. The microarray is located in the center and 10 mm from the top of the glass substrate slide on the barcoded side. For additional help with side orientation, the 1.4 mm cropped corner of the glass substrate (“chamfer”) should be located in the upper right corner of the glass substrate when the barcode is facing upward. Powder free nitrile gloves should be worn at all time while handling microarrays to avoid the transfer of dust, debris, hand oils, and other contaminants that can interfere with hybridization and detection. ArrayIt® Microarray Cleanroom Gloves provide superior hand protection for microarray applications. The recommended probe sample volume for Pathways™ Microarrays is 20 µl, which will form a 60 µm sample layer on the microarray when using a 18 x 18 mm lifter type cover slip. The recommended hybridization buffer is HybIt® 2 Hybridization Solution. Arrayit provides an affordable and widely used line of Hybridization Cassettes and these work very well for low to medium throughput applications. Users may also employ the Arrayit TrayMix™ S4 Automated Hybridization Stations to allow automated reactions and washing. Probe mixtures can be generated from cloned or cellular DNA, RNA or protein, and dozens of fluorescent and non-fluorescent labeling schemes have been described in the literature. For gene expression applications with Pathways™ Microarrays use 0.5 µg of amino-allyl labeled cDNA per color (1.0 µg total of green and red probe for two-color experiments) is recommended to produce strong hybridization signals. Probe mixtures should be spun at 18,000 x g for 1 min to pellet debris prior to use. The microarray substrate slide, hybridization cassette or chamber, lifter slip and probe mixture should be pre-heated to 42°C for 5 min prior to hybridization to ensure low and uniform background. Pipette the 20 µl probe mixture directly onto the center of the microarray and quickly place the lifter slip onto the probe droplet, allowing the probe mixture to sheet evenly across the surface. Probe mixtures should be reacted for 3.5 hours at 42°C for gene expression applications, though different times and temperatures can be used for probe mixtures of different concentration and complexity. Probes derived from cloned and recombinant sources will probably produce satisfactory results within 5-10 minutes because the molecular complexity of such sources is low and Pathways™ Microarrays performance is extremely high. Probe mixtures derived from complex sources such as human genomic DNA, mRNA and protein may require more than 3.5 hours to produce strong signals. In rare cases, users may opt for up to 18 hour reaction times, though probe drying and elevated background noise can become an issue with prolonged hybridizations. After the hybridization step is complete, users should proceed quickly to the wash steps to prevent probe drying and elevated background.

3. Wash microarrays to remove unbound probe molecules. After the probe binding reaction is complete, the microarray must be washed to remove unbound material. Oligonucleotide-DNA or oligonucleotide-RNA hybrids are typically washed at high-stringency to remove labeled probe molecules that have bound weakly or non-specifically. Lesser stringency can be use in cases where DNA and mRNA probe mixtures are derived from homologous species such as primate, mouse and rat. For protein binding applications, oligonucleotide-protein complexes should be washed to remove proteins that have bound weakly or non-specifically. The oligonucleotides on Pathways™ Microarrays are bound covalently to the glass substrate, and therefore the oligonucleotides themselves are stable to elevated temperature (≤70°C), low salt, and other neutral pH (pH 6-8) treatments. Users should avoid extremes in temperature and pH to avoid damaging the oligonucleotides. Protein experiments with H25K typically make use of reactions 20-37°C and washes with phosphate-buffered saline (PBS) buffers containing dilute additions of detergent (e.g. 0.1-0.01% TritonX100). Prior to washing, the lifter slip must be removed from the microarray surface to allow efficient washing and to avoid scratching the spots on the surface. For gene expression applications, quickly remove the lifter slip by submerging microarray for 5-10 sec in 42°C in Wash Buffer A and wash as follows: 5 min at 42°C in Wash Buffer A, 5 min at room temperature in Wash Buffer B, and 1 min at room temperature in Wash Buffer C, followed by a 1 sec dunk in dH2O. Washes should be performed in High-Throughput Wash Stations with moderate mixing or in the TrayMix™ S4 Hybridization Station. Users can experiment with different wash buffers and different temperatures (20-65°C) to optimize the washing process for a particular application. Following the final wash step, microarrays should be dried quickly by centrifugation for 3 sec in an Arrayit Microarray High-Speed Centrifuge in preparation for scanning.

4. Detect signals by scanning or imaging. Pathways™ Microarrays should be inserted into a microarray scanning or imaging device to acquire the signals. The Arrayit InnoScan® 710 and 910 Fluorescence Scanners and the highly affordable Arrayit SpotLight™ Microarray Fluorescence Scanner works well for this application, though all Pathways™ Microarrays are compatible with all major brands of microarray glass microscope slide microarray scanners. Scanners and imagers should be capable of at least 10 µm resolution, the lowest recommended resolution setting. Scanning at 3-5 µm resolution produces higher quality images. The selected scan area should be large enough to capature all spots in a single image. In addition to the resolution and scan area settings, correct settings should be used for wavelength(s), gain, laser intensity, PMT, and other technical parameters required to produce high quality, data-rich images. For expression analysis applications involving complex mixtures of mRNA varying greatly in concentration, multiple scanner settings (e.g. low and high sensitivity) across two or more images can be used to capture signals spanning up to 6 orders of magnitude. Multi-color experimentation involving, for example, cyanine 3 and cyanine 5 labels, obviously require data capture at two different wavelengths (e.g. 532 and 635 nm). Images should be saved as 16-bit TIFF files for quantification and downstream analysis.

5. Quantify the signals. Arrayit MAPIX® and ImageGene quantification software is highly recommended for quantification. Use GAL file spot map provided with the microarrays. Place grid over the file, making sure that all subgrids and spots are properly aligned. MAPIX® quantification settings should be set to allow the software to find all of the printed spots in an automated manner. The following settings work well for most applications: Max position offset (% pitch) 30, Min. diameter (% Dth) 80, Max. diameter (% Dth) 190, S/B border width (pixels) 1.6 and Background diameter (pitch) 1.6. Once the grid has been accurately aligned, the quantification process should be initiated to extract data values from H25K using the photmetric calculation command. The extracted data should be saved and then opened in Microsoft Excel or some other software program for downstream analysis.

6. Perform data analysis. Properly quantified images will yield great data. Values should be analyzed using a variety of inclusion and exclusion criteria to ensure statistical relevance of all values. For most applications, background-subtracted median signal intensities provide the most accurate reporting of spot signal intensity. Data from multiple images can be compared to identify up- and down-regulated genes in expression profiling experiments. Arrayit offers highly sophisticated Genome Pathways Builder™ software analysis for Biomarker Discovery and Validation Services customers. Genome Pathways Builder™ constructs biological Pathwayss from vast quantities of expression data, providing customers with a detailed molecular understanding of a disease process. Genome Pathways Builder™ also allows pharmaceutical clients to understand the mode of drug action as well as off-targets as a means of benchmarking pharmaceutical leads prior to and during clinical trials.

Technical Assistance
Please contact us if you have any questions, comments, suggestions, or if you need technical assistance. By electronic mail, use arrayit@arrayit.com and type "H25K technical assistance" into the subject line. By email, arrayit@arrayit.com between the hours of 8AM and 8PM PST Monday through Friday. We want to hear about your successes and are always happy to publish contributed H25K sample data on our website.

neurotransmitter-microarray
Figure 3. Arrayit Neurotransmitter Receptors and Regulators Pathways Microarrays (Cat. NRPM) enable targeted gene expression analysis of neurotransmitter signaling in human cells.

Troubleshooting Tips
1. Weak signals

  • Degraded DNA, RNA or protein. Check sample integrity.
  • Poor sample labeling efficiency. Check probe mixture.
  • Wash stringency too high.

2. High background fluorescence

  • Probe mixture drying out during hybridization. Keep hydrated for duration.
  • Probe drying after hybridization. Transfer quickly to wash steps.
  • Probe volume too low. Use ≥50.0 µl for H25K.
  • Purify probe mixture to remove contaminating unincorporated fluors.
  • Hybridization time too long. Use a maximum time of 6 hrs.
  • Poor washing procedure. Increase time or stringency.

3. Low frequency of differentially expressed genes (gene expression)

  • Wash stringency too low. Reduce salt and/or increase temperature.
  • Messenger RNA samples highly similar. Check samples/stimuli.

4. Artifacts observed in two-color experiments (gene expression)

  • Dye effects to blame. Swap colors and re-examine data.

Recommended Equipment

Ordering Information

Product

Description

Catalog ID

Amino Acid Metabolism I

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human amino acid metabolism I pathway, 10 microarrays per kit.

AA1PM

Amino Acid Metabolism II

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human amino acid metabolism II pathway, 10 microarrays per kit.

AA2PM

Adipogenesis

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human adipogenesis pathway, 10 microarrays per kit.

ADPM

Angiogenic Growth Factors and Angiogenesis Inhibitors

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human angiogenic growth factors and angiogenesis inhibitors pathway, 10 microarrays per kit.

AGPM

Angiogenesis

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human angiogenesis pathway, 10 microarrays per kit.

ANPM

Antiviral Response

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human antiviral response pathway, 10 microarrays per kit.

ARPM

Apoptosis

Arrayit Pathways Focused Human Gene Expression Microarrays containing 375 gene targets for the human apoptosis pathway, 10 microarrays per kit.

ASPM

Atherosclerosis

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human atherosclerosis pathway, 10 microarrays per kit.

ATPM

Autophagy

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human autophagy pathway, 10 microarrays per kit.

AUPM

Alzheimer's Disease

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human Alzheimer's Disease pathway, 10 microarrays per kit.

AZPM

Brain Cancer miRNA

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human brain cancer miRNA pathway, 10 microarrays per kit.

BCPM

Breast Cancer and Estrogen Receptor Signaling

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human breast cancer and estrogen receptor signaling pathway, 10 microarrays per kit.

BEPM

Breast Cancer

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human breast cancer pathway, 10 microarrays per kit.

BRPM

cAMP/Ca2+ Signaling Pathways Discovery

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human cAMP/Ca2+ signaling pathways discovery pathway, 10 microarrays per kit.

CAPM

Cell Cycle

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human cell cycle pathway, 10 microarrays per kit.

CCPM

Cell Development and Differentiation miRNA

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human cell development and differentiation miRNA pathway, 10 microarrays per kit.

CDPM

Cell Surface Markers

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human cell surface markers pathway, 10 microarrays per kit.

CEPM

Chemokines and Receptors

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human chemokines and receptors pathway, 10 microarrays per kit.

CHPM

Cytoskeleton Regulators

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human cytoskeleton regulators pathway, 10 microarrays per kit.

CKPM

Cell Lineage Identification

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human cell lineage identification pathway, 10 microarrays per kit.

CLPM

Cancer miRNA

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human cancer miRNA pathway, 10 microarrays per kit.

CMPM

Cancer Drug Resistance and Metabolism

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human cancer drug resistance and metabolism pathway, 10 microarrays per kit.

CNPM

Common Cytokines

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human common cytokines pathway, 10 microarrays per kit.

COPM

Cancer Pathways Discovery

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human cancer pathways discovery pathway, 10 microarrays per kit.

CPPM

Cardiotoxicity

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human cardiotoxicity pathway, 10 microarrays per kit.

CRPM

Cellular Senescence

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human cellular senescence pathway, 10 microarrays per kit.

CSPM

Cancer Drug Targets

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human cancer drug targets pathway, 10 microarrays per kit.

CTPM

Cell Motility

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human cell motility pathway, 10 microarrays per kit.

CYPM

Dendritic and Antigen Presenting Cell

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human dendritic and antigen presenting cell pathway, 10 microarrays per kit.

DAPM

DNA Damage Signaling

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human DNA damage signaling pathway, 10 microarrays per kit.

DDPM

Drug Metabolism: Phase I Enzymes

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human drug metabolism phase I enzymes pathway, 10 microarrays per kit.

DM1PM

Drug Metabolism: Phase II Enzymes

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human drug metabolism phase II enzymes pathway, 10 microarrays per kit.

DM2PM

Drug Metabolism

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human drug metabolism pathway, 10 microarrays per kit.

DMPM

Diabetes

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human diabetes pathway, 10 microarrays per kit.

DPM

DNA Repair

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human DNA repair pathway, 10 microarrays per kit.

DRPM

Drug Transporters

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human drug transporters pathway, 10 microarrays per kit.

DTPM

Extracellular Matrix and Adhesion Molecule

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human extracellular matrix and adhesion molecule pathway, 10 microarrays per kit.

EAPM

Epigenetic Chromatin Modification Enzymes

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human epigenetic chromatin modification enzymes pathway, 10 microarrays per kit.

ECPM

Endothelial Cell Biology

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human endothelial cell biology pathway, 10 microarrays per kit.

ENPM

EGF/PDGF Signaling

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human EGF/PDGD signaling pathway, 10 microarrays per kit.

EPPM

Epigenetic Chromatin Remodeling Factors

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human epigenetic chromatin remodeling factors pathway, 10 microarrays per kit.

ERPM

Embryonic Stem Cells

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human embryonic stem cells pathway, 10 microarrays per kit.

ESPM

Epithelial to Mesenchymal Transition (EMT)

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human epithelial to mesenchymal transition (EMT) pathway, 10 microarrays per kit.

ETPM

Fatty Acid Metabolism

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human fatty acid metabolism pathway, 10 microarrays per kit.

FAPM

Fibrosis

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human fibrosis pathway, 10 microarrays per kit.

FIPM

Gastric Cancer Methylation

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human gastric cancer methylation pathway, 10 microarrays per kit.

GCPM

Growth Factors

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human growth factors pathway, 10 microarrays per kit.

GFPM

Glucose Metabolism

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human glucose metabolism pathway, 10 microarrays per kit.

GMPM

GPCR Signaling

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human GPCR signaling pathway, 10 microarrays per kit.

GPPM

G Protein Coupled Receptors

Arrayit Pathways Focused Human Gene Expression Microarrays containing 375 gene targets for the human G protein couples receptors pathway, 10 microarrays per kit.

GRPM

Glycosylation

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human glycosylation pathway, 10 microarrays per kit.

GSPM

Huntington's Disease

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human Huntington’s Disease pathway, 10 microarrays per kit.

HDPM

Hedgehog Signaling

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human hedgehog signaling pathway, 10 microarrays per kit.

HEPM

Housekeeping Genes

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human housekeeping genes pathway, 10 microarrays per kit.

HGPM

HIV Infection and Host Response

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human HIV infection and host response pathway, 10 microarrays per kit.

HIPM

Hematopoietic Stem Cells and Hematopoiesis

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human hematopoietic stem cells and hematopoiesis pathway, 10 microarrays per kit.

HMPM

Homeobox (HOX) Genes

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human homeobox (HOX) genes pathway, 10 microarrays per kit.

HOPM

Heat Shock Proteins

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human heat shock proteins pathway, 10 microarrays per kit.

HSPM

Hypertension

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human hypertension pathway, 10 microarrays per kit.

HTPM

Hypoxia Signaling

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human hypoxia signaling pathway, 10 microarrays per kit.

HYPM

Innate and Adaptive Immune Response

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human innate and adaptive immune response pathway, 10 microarrays per kit.

IAPM

Inflammatory Cytokines and Receptors

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human inflammatory cytokines and receptors pathway, 10 microarrays per kit.

ICPM

Interferon Response

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human interferon response pathway, 10 microarrays per kit.

IFPM

Immunopathology

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human immunopathology pathway, 10 microarrays per kit.

IMPM

Inflammasomes

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human inflammasomes pathway, 10 microarrays per kit.

INPM

Inflammatory Response and Autoimmunity

Arrayit Pathways Focused Human Gene Expression Microarrays containing 375 gene targets for the human inflammatory response and autoimmunity pathway, 10 microarrays per kit.

IRPM

Insulin Signaling

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human insulin signaling pathway, 10 microarrays per kit.

ISPM

Interferons and Receptors

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human interferons and receptors pathway, 10 microarrays per kit.

ITPM

JAK/STAT Signaling

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human JAK/STAT signaling pathway, 10 microarrays per kit.

JSPM

Liver Cancer

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human liver cancer pathway, 10 microarrays per kit.

LCPM

Leukemia and Lymphoma

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human Leukemia and Lymphoma pathway, 10 microarrays per kit.

LLPM

Lipoprotein Signaling and Cholesterol Metabolism

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human lipoprotein signaling and cholesterol metabolism pathway, 10 microarrays per kit.

LSPM

Lung Cancer Methylation

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human lung cancer methylation pathway, 10 microarrays per kit.

LUPM

Mitochondrial Energy Metabolism

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human mitochondrial energy metabolism pathway, 10 microarrays per kit.

MEPM

miRNA Human Discovery

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human miRNA human discovery pathway, 10 microarrays per kit.

MIPM

MAP Kinase Signaling

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human MAP kinase signaling pathway, 10 microarrays per kit.

MKPM

Melanoma Methylation

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human melanoma methylation pathway, 10 microarrays per kit.

MMPM

Mesenchymal Stem Cells

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human mesenchymal stem cells pathway, 10 microarrays per kit.

MNPM

mTOR Signaling

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human mTOR signaling pathway, 10 microarrays per kit.

MRPM

Multiple Sclerosis

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human multiple sclerosis pathway, 10 microarrays per kit.

MSPM

Mitochondria

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human mitochondria pathway, 10 microarrays per kit.

MTPM

Molecular Toxicology Pathways Discovery

Arrayit Pathways Focused Human Gene Expression Microarrays containing 375 gene targets for the human molecular toxicology pathways discovery pathway, 10 microarrays per kit.

MXPM

Neurological Development and Disease

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human neurological development and disease pathway, 10 microarrays per kit.

NDPM

NFkB Signaling

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human NFkB signaling pathway, 10 microarrays per kit.

NFPM

Neuroscience Ion Channels and Transporters

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human neuroscience ion channels and transporters pathway, 10 microarrays per kit.

NIPM

Neurogenesis and Neural Stem Cells

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human neurogenesis and neural stem cells pathway, 10 microarrays per kit.

NNPM

Nitric Oxide Signaling

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human nitric oxide signaling pathway, 10 microarrays per kit.

NOPM

Neurotrophins and Receptors

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human neurotrophins and receptors pathway, 10 microarrays per kit.

NPPM

Neurotransmitter Receptors and Regulators

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human neurotransmitter receptors and regulators pathway, 10 microarrays per kit.

NRPM

Notch Signaling

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human notch signaling pathway, 10 microarrays per kit.

NSPM

Nephrotoxicity

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human nephrotoxicity pathway, 10 microarrays per kit.

NTPM

Nuclear Receptors and Coregulators

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human nuclear receptors and coregulators pathway, 10 microarrays per kit.

NUPM

Neurotoxicity

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human neurotoxicity pathway, 10 microarrays per kit.

NXPM

Obesity

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human obesity pathway, 10 microarrays per kit.

OBPM

Oncogenes and Tumor Suppressor Genes

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human oncogenes and tumor suppressor genes pathway, 10 microarrays per kit.

ONPM

Osteogenesis

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human osteogenesis pathway, 10 microarrays per kit.

OSPM

Oxidative Stress and Antioxidant Defense

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human oxidative stress and antioxidant defense pathway, 10 microarrays per kit.

OXPM

PI3K- AKT Signaling

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human PI3K- AKT Signaling.

P13PM

p53 Signaling

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human p53 signaling pathway, 10 microarrays per kit.

P53PM

Prostate Cancer Methylation

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human prostate cancer methylation pathway, 10 microarrays per kit.

PCPM

Parkinson's Disease

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human Parkinson’s Disease pathway, 10 microarrays per kit.

PDPM

Polycomb and Trithorax Complexes

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human polycomb and trithorax complexes pathway, 10 microarrays per kit.

PLPM

Protein Phosphatases

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human protein phosphatases pathway, 10 microarrays per kit.

PPPM

Primary Cilia

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human primary cilia pathway, 10 microarrays per kit.

PRPM

Polycomb and Trithorax Target Genes

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human polycomb and trithorax target genes pathway, 10 microarrays per kit.

PTPM

Stem Cell Signaling

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human stem cell signaling pathway, 10 microarrays per kit.

SCPM

Stem Cell Transcription Factors

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human stem cell transcription factors pathway, 10 microarrays per kit.

SFPM

Signal Transduction Pathways Discovery

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human signal transduction pathways discovery pathway, 10 microarrays per kit.

SIPM

Skeletal Muscle Myogenesis and Myopathy

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human skeletal muscle myogenesis and myopathy pathway, 10 microarrays per kit.

SKPM

Serum miRNA

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human serum miRNA pathway, 10 microarrays per kit.

SMPM

Synaptic Plasticity

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human synaptic plasticity pathway, 10 microarrays per kit.

SPPM

Stress Response to Cellular Damage

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human stress response to cellular damage pathway, 10 microarrays per kit.

SRPM

Stem Cell

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human stem cell pathway, 10 microarrays per kit.

STPM

Stress and Toxicity Pathways Discovery

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human stress and toxicity pathways discovery pathway, 10 microarrays per kit.

SXPM

T Cell Anergy and Immune Tolerance

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human T cell anergy and immune tolerance pathway, 10 microarrays per kit.

TAPM

T Cell and B Cell Activation

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human T cell and B cell activation pathway, 10 microarrays per kit.

TBPM

T Cell Activation Methylation

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human T cell activation methylation pathway, 10 microarrays per kit.

TCPM

Terminal Differentiation Markers

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human terminal differentiation markers pathway, 10 microarrays per kit.

TDPM

T Helper Cell Differentiation

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human T helper cell differentiation pathway, 10 microarrays per kit.

TEPM

Transcription Factors

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human transcription factors pathway, 10 microarrays per kit.

TFPM

TGFß/BMP Signaling

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human TGFß/BMP signaling pathway, 10 microarrays per kit.

TGPM

Th17 for Autoimmunity and Inflammation

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human Th17 for autoimmunity and inflammation pathway, 10 microarrays per kit.

THPM

Telomeres and Telomerase

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human telomeres and telomerase pathway, 10 microarrays per kit.

TLPM

Tumor Necrosis Factor (TNF) Ligands and Receptors

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human tumor necrosis factor (TNF) ligands and receptors pathway, 10 microarrays per kit.

TNPM

Toll Like Receptor Signaling

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human toll like receptor signaling pathway, 10 microarrays per kit.

TRPM

Tumor Suppressor Methylation

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human tumor suppressor methylation pathway, 10 microarrays per kit.

TSPM

Th1-Th2-Th3

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human Th1-Th2-Th3 pathway, 10 microarrays per kit.

TTPM

Tumor Metastasis

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human tumor metastasis pathway, 10 microarrays per kit.

TUPM

Ubiquitination (Ubiquitylation)

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human ubiquitination (ubiquitylation) pathway, 10 microarrays per kit.

UBPM

Unfolded Protein Response

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human unfolded protein response pathway, 10 microarrays per kit.

UNPM

VEGF Signaling

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human VEGF signaling pathway, 10 microarrays per kit.

VEPM

Wound Healing

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human wound healing pathway, 10 microarrays per kit.

WHPM

WNT Signaling

Arrayit Pathways Focused Human Gene Expression Microarrays containing 89 gene targets for the human WNT signaling pathway, 10 microarrays per kit.

WNPM

 

Storage Conditions

Arrayit's DNA Microarrays are manufactured, processed and packaged in advanced cleanrooms facilities and are stable at room temperature (25°C) for 6 months from the date of receipt. Unprocessed microarrays from Arrayit have an indefinite shelf life. If you wish to receive your microarrays unprocessed, please specify this at the time of ordering. Store in the sealed anti-static shipping envelope and avoid exposure to elevated temperature, humidity, and dust until use.

 

Warranty

Arrayit life sciences products are sold for research purposes only. Arrayit brand products have been scientifically developed and are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All Arrayit brand products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any performance issues should be reported to Arrayit immediately. Arrayit’s liability is limited to the replacement of the product, or a full refund. Any misuse of this product is the full responsibility of the user, and Arrayit makes no warranty or guarantee under such circumstances. Pricing may vary up to 30% due to costs associated with distribution, import taxes, duties, customs clearance and shipping.

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