Microarray Hybridization Cassette Silicone Replacement Gasket GAHC1X16

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Item Number: GAHC1X16

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Arrayit Hybridization Cassette Silicone Replacement Gasket GAHC1X16 allowing the demarcation of a 2 x 8 configuration of wells at 9 mm centers. This replacement gasket is used 1 each with the Arrayit Hybridization Cassette AHC1X16 (Cat. AHC1X16) and 4 each with the Arrayit Hybridization Cassette AHC4X16 (Cat. AHC4X16).

Microarray Tools - Hybridization Cassettes - Microarray Multi-Well Hybridization Cassettes and Silicone Replacement Gaskets

hybridization-microarray.11 Arrayit offers innovative hardware solutions for creating multi-well microarrays on single glass substrate slides (1 x 25 x 76 mm) for rapid testing of many patient research specimens at once. Reaction cassettes containing 8, 16, 24, 48 or 96 wells utilize silicone gaskets to create individual reaction wells encompassing the printed microarrays on the glass slides. The upper portion of the Hybridization Cassette allows the introduction of individual samples, making these cassettes ideal for DNA and protein microarray research and high-throughput testing. The AHC4X8L Hybridization Cassette (Cat. AHC4X8L) shown above has a 150-300 µl well volume. Widely used cassettes such as AHC4X16 and AHC4X24 have smaller wells and permit the use of smaller sample volumes.

Microarray-Hybridization-1X24

Microarray-Hybridization_1x16

Figure 1A. Arrayit Hybridization Cassette AHC1X24 holds one standard glass slide (1 x 25 x 76 mm) and has 7.5 x 6.5 mm round wells spaced at 9 mm centers in a 3 x 8 format for easy loading of the 24 wells with multi-channel pipettes and automation systems. Note that the proper orientation fits the wide portion of the silicone gasket against the hardware and the thin portion against the glass substrate slide.

Figure 1B. Arrayit Hybridization Cassette AHC1X16 holds one standard glass slide (1 x 25 x 76 mm) and has 7.5 x 7.5 mm square wells spaced at 9 mm centers in a 2 x 8 format for easy loading of the 16 wells with multi-channel pipettes and automation systems. Note that the proper orientation fits the wide portion of the gasket against the hardware and the thin portion against the glass substrate slide.

microarray-slide-reaction-areas
Figure 2. Diagramatic depiction of the wells formed by Arrayit Hybridization Cassette AHC1X24 (left) and AHC1X16 (right), which both demarcate wells on standard microarray substrate slides (1 x 25 x 76 mm). Use these spacing coordinates to program the microarrayer prior to printing the 3 x 8 (24 microarrays per slide) or 2 x 8 (16 microarrays per slide) microarray patterns required to utilize these multi-well Hybridization Cassettes. Use the SpotBot and NanoPrint Microarrayer to simplify the microarray manufacturing using these formats. Please see Ordering Information below for cassettes and gaskets with larger reaction areas.

Microarray-Mybridization-AHC4x16
Figure 3. Arrayit Hybridization Cassette AHC4X16 (Cat. AHC4X16) is identical to Hybridization Cassette AHC1X16 except that it contains four identical units allowing the use of four microarray glass substrate slides (1 x 25 x 76 mm). Arrayit Hybridization Cassette AHC4X16 has a 7.5 x 7.5 mm well size set at 9 mm centers in four identical 2 x 8 units for a total of 64 wells that can be used to process 64 microarrays with 64 different patient research specimens for high-throughput testing. Well openings in the top portion of Hybridization Cassette AHC4X16 (shown here) are 5.5 x 5.5 mm. Requires the use of four individual silicone gaskets (Cat. GAHC1X16) to demarcate the 64 individual wells, and the four gaskets are included with the purchase of the AHC4X16 product. Easily sealed with foil seals to prevent evaporation during use.

microarary-AHC4x24
AHC4x24-Microarray

Figure 4. Arrayit Hybridization Cassette AHC4X24 (Cat. AHC4X24) is identical to AHC1X24 except that it contains four identical 24-well units, allowing the use of four glass substrate slides (1 x 25 x 76 mm). AHC4x24 has a 7.5 x 6.5 mm well size set at 9 mm centers in four identical 3 x 8 units for a total of 96 wells that can be used to process 96 microarrays with 96 different patient research specimens for high-throughput testing. Well openings in the top portion of the cassette are 5.5 x 5.5 mm. The AHC4X24 requires the use of a silicone gasket (Cat. GAHC4X24) to demarcate the individual wells. The GAHC4X24 silicone gasket is included with the purchase of the AHC4X24 cassette. The top of the cassette is easily sealed with foil seals to prevent evaporation.

arrayit_microarray_gaskets
Figure 5. Arrayit Silicone Replacement Gaskets for multi-well microarray Hybridization Cassettes. Use Replacement Gasket GAHC1X16 (upper left) with Hybridization Cassette AHC1X16, Replacement Gasket GAHC1X24 (upper right) with Hybridization Cassette AHC1X24, Replacement Gasket GAHC1X16 four each (lower left) with Hybridization Cassette AHC4X16, and one Replacement Gasket GAHC4X24 (lower right) with Hybridization Cassette AHC4X24. To prevent leakage during use, gaskets must be used in the proper orientation with the wide portion placed against the hybridization cassette top and the thin portion placed against the glass substrate slide (1 x 25 x 76 mm). Note: Arrayit offers a variety of gaskets with larger reaction areas as shown in Ordering Information below.

AHC4X8S
Figure 6. Schematic drawing of Arrayit Hybridization Cassette AHC4X8S (Cat. AHC4X8S). AHC4X8S holds 4 glass substrate slides (1 x 25 x 76 mm) and four identical GAHC4X8S silicone gaskets define the 4 x 8 wells (32 wells total), with the 8 wells down the slide spaced at 9 mm centers. Each of the 32 wells in the AHC4X8S cassette are 17 mm wide. For larger wells, use Cat. ACH4X8L (see Fig. 7).

AHC4X8L
Figure 7. Schematic drawing of Arrayit Hybridization Cassette AHC4X8L (Cat. AHC4X8L). AHC4X8L holds 4 glass substrate slides (1 x 25 x 76 mm) and a single GAHC4X8L silicone gasket defines the 8 x 4 wells (32 wells total), with the 8 wells down the slide spaced at 9 mm centers. Each of the 32 wells in the AHC4X8L cassette are 23 mm wide. For smaller wells, use Cat. ACH4X8S (see Fig. 6).

AHC4X24
Figure 8. Schematic drawing of Arrayit Hybridization Cassette AHC4X24 (Cat. AHC4X24). AHC4X24 holds 4 glass substrate slides (1 x 25 x 76 mm) and the GAHC4X24 silicone gasket defines the 4 x 24 wells (96 wells total), with the 24 wells down spaced at 9 mm centers.

hybridization-mixing
Figure 9. Arrayit Array Plate Microarray Hybridization Stations (Cat. MMHS110V and MMHS220V) are available for use with Arrayit Hybridization Cassettes AHC4X8, AHC4X16 and AHC4X24. When ordering the MMHS110V and MMHS220V Hybridization Stations, make sure to specify which hybridization cassettes you will be using to make sure we include the correct instrument heating and cooling block. Heating and cooling blocks are easily switched using the four mounting screws in the corners of the blocks. Reaction cassettes are easily sealed with foil seals to prevent evaporation during long reactions. Wash using Microarray Wash Trays and a plate shaker.

Technical Assistance

Please contact us if you have any comments, suggestions, or if you need technical assistance. We can be reached at all hours by electronic mail (arrayit@arrayit.com) from Monday-Friday 8:00 AM-8 PM PST. Please remember that we want to hear about your successes!

Troubleshooting Tips

Slides not seating correctly in the base - use a fine forceps to press down on the end of the slide when tightening the locating screw.

Slides stick to the gasket when opening the cassette – gently re-tighten the slide locating screws after use before opening the cassette.

Scientific Publications

Click here and here for Arrayit cassette scientific publications.

Ordering Information

Product	Description	Catalog ID
Arrayit Multi-Well Hybridization Cassette, AHC1X16	Arrayit Multi-Well Hybridization Cassette AHC1X16 contains a base for the slide, 16-well silicone gasket and a top portion the holds the gasket. The AHC1X16 cassette has a total of 16 wells that are 7.5 x 7.5 mm in size and configured in a 2 x 8 pattern at 9 mm centers, with a capacity for one glass substrate slide (1 x 25 x 76 mm). The well openings on the top of the cassette are 5.5 mm x 5.5 mm. The hybridization cassette utilizes a compression-fit silicone gasket to separate the microarray reaction areas, eliminating the need for contaminating adhesives and inks. The cassette is made of durable, flat, precision-machined aluminum to ensure proper alignment and a leak-proof fit. The top of the cassette is embossed with a 1-2 number panel across the top and an A-H letter panel down the left side for easy identification of each of the 16 wells. Each AHC1X16 cassette includes an anodized aluminum top and bottom, square cassette wells, a 2 x 8 well silicone gasket and no septa mat for sealing the wells during use. Wells can be sealed with adhesive foil seals (Cat. MPAFS) if necessary.	AHC1X16
Arrayit Multi-Well Hybridization Cassette, AHC1X24	Arrayit Multi-Well Hybridization Cassette AHC1X24 contains a base for the slide, 24-well silicone gasket and a top portion that holds the gasket. The cassette has a total of 24 wells that are 7.5 x 6.5 mm in size and configured in a 3 x 8 pattern at 9 mm centers, with a capacity for one glass substrate slide (1 x 25 x 76 mm). The AHC1X24 cassette utilizes a compression-fit silicone gasket to separate the microarray reaction areas, eliminating the need for contaminating adhesives and inks. The 7.5 mm x 6.5 mm reaction areas are configured at 9 mm center-to-center spacing in the 3 x 8 format for compatibility with all multi-channel pipettes and automated liquid handling robots and workstations. AHC1X24 is precision-machined from durable, flat high-grade aluminum to ensure proper alignment and a leak-proof fit. The top portion of the cassette is engraved with a 1-3 number panel across the top and an A-H letter panel down the left side for unambiguous identification of each of the 24 subgrids. A silicone septa mat is used to seal the loading ports to prevent evaporation during use. Each AHC1X24 cassette includes an anodized aluminum top and bottom, round cassette wells, a 3 x 8 well silicone gasket, and a septa mat for sealing the wells during use.	AHC1X24
Arrayit Multi-Well Hybridization Cassette, AHC4X16	Arrayit Multi-Well Hybridization Cassette AHC4X16 contains a base for the four slides, four 16-well silicone gaskets and a top portion that holds the four silicone gaskets. The cassette has a total of 64 wells that are 7.5 x 7.5 mm in size and configured in four 2 x 8 patterns at 9 mm centers, with a capacity for four glass substrate slides (1 x 25 x 76 mm). The AHC4X16 cassette utilizes a compression-fit silicone gasket to separate the microarray reaction areas, eliminating the need for contaminating adhesives and inks. The 7.5 mm x 7.5 mm reaction areas are compatible with automatic pipettors and liquid handling systems. Well openings in the cassette top are 5.5 x 5.5 mm for the easy introduction of wash buffers, labeling reagents and other materials. AHC4X16 cassettes are manufactured from durable, flat, precision-machined aluminum to ensure proper alignment and a leak-proof fit. Arrayit offers cassette customization to allow for the use of glass slides from other manufacturers. Please contact us at arrayit@arrayit.com for customization pricing. The top of the cassettes are embossed with a letter and number for easy identification of each microarray position in the 2x8 configuration. Highly recommended for DNA and protein microarray multiplex assays. Use gasket GAHC4X16 as the replacement silicone gaskets.	AHC4X16
Arrayit Multi-Well Hybridization Cassette, AHC4X24	Arrayit Multi-Well Hybridization Cassette AHC4X24 contains a base, a 4 x 24-well single silicone gasket and a top portion that holds the gasket. The cassette has a total of 96 wells that are 7.5 x 6.5 mm in size and configured four 3 x 8 patterns at 9 mm centers, with a capacity for four glass substrate slides (1 x 25 x 76 mm). The AHC4X24 cassette utilizes a compression-fit silicone gasket to separate the microarray reaction areas, eliminating the need for contaminating adhesives and inks. The 7.5 mm x 6.5 mm reaction areas are compatible with all multi-channel pipettes and automated liquid handling robots and workstations. AHC4X24 is precision-machined from durable, flat high-grade aluminum to ensure proper alignment and a leak-proof fit. Arrayit offers cassette customization to allow for the use of glass slides from other manufacturers. Please contact us at arrayit@arrayit.com for customization pricing. The top portion of the cassette is engraved with a 1-12 number panel across the top and an A-H letter panel down the left side for unambiguous identification of each of the 96 reaction wells. Each AHC4X24 cassette includes an anodized aluminum top and bottom, square cassette wells, SBS compliant top and bottom, a 3 x 8 x 4 well silicone gasket and no septa mat for sealing the wells during use. Wells can be sealed with adhesive foil seals (Cat. MPAFS) if necessary.	AHC4X24
Arrayit Multi-Well Hybridization Cassette, AHC4X8S	Arrayit Multi-Well Hybridization Cassette AHC4X8S (S = smaller wells) contains a base, four 1 x 8-well silicone gaskets and a top portion that holds the four gaskets. The cassette has a total of 32 wells that are 7.5 x 17 mm in size and configured four 1 x 8 patterns at 9 mm centers down the cassette, with a capacity for four glass substrate slides (1 x 25 x 76 mm). The AHC4X8S cassette utilizes four compression-fit silicone gaskets to separate the microarray reaction areas, eliminating the need for contaminating adhesives and inks. The 7.5 mm x 17 mm reaction areas have a 100-200 µl well volume and are compatible with all multi-channel pipettes and automated liquid handling robots and workstations pipetting down the cassette. AHC4X8S is precision-machined from durable, flat high-grade aluminum to ensure proper alignment and a leak-proof fit. Arrayit offers cassette customization to allow for the use of glass slides from other manufacturers. Please contact us at arrayit@arrayit.com for customization pricing. The top portion of the cassette is engraved with a 1-12 number panel across the top and an A-H letter panel down the left side for unambiguous identification of each of the 32 reaction wells. Each AHC4X8S cassette includes an anodized aluminum top and bottom, square cassette wells, SBS compliant top and bottom, four 1 x 8 well silicone gaskets and no septa mat for sealing the wells during use. Wells can be sealed with adhesive foil seals (Cat. MPAFS) if necessary.	AHC4X8S
Arrayit Multi-Well Hybridization Cassette, AHC4X8L	Arrayit Multi-Well Hybridization Cassette AHC4X8L (L = larger wells) contains a base, a 4 x 8-well single silicone gasket and a top portion that holds the gasket. The cassette has a total of 32 wells that are 7.5 x 23 mm in size and configured four 1 x 8 patterns at 9 mm centers down the cassette, with a capacity for four glass substrate slides (1 x 25 x 76 mm). The AHC4X8L cassette utilizes a compression-fit silicone gasket to separate the microarray reaction areas, eliminating the need for contaminating adhesives and inks. The 7.5 mm x 23 mm reaction areas have a 150-300 µl well volume are compatible with all multi-channel pipettes and automated liquid handling robots and workstations pipetting down the cassette. AHC4X8L is precision-machined from durable, flat high-grade aluminum to ensure proper alignment and a leak-proof fit. Arrayit offers cassette customization to allow for the use of glass slides from other manufacturers. Please contact us at arrayit@arrayit.com for customization pricing. The top portion of the cassette is engraved with a 1-12 number panel across the top and an A-H letter panel down the left side for unambiguous identification of each of the 32 reaction wells. Each AHC4X8L cassette includes an anodized aluminum top and bottom, square cassette wells, SBS compliant top and bottom, a 1 x 8 x 4 well silicone gasket and no septa mat for sealing the wells during use. Wells can be sealed with adhesive foil seals (Cat. MPAFS) if necessary.	AHC4X8L
Silicone Replacement Gasket GAHC1X16	Arrayit Silicone Replacement Gasket GAHC1X16 is for Arrayit Multi-Well Hybridization Cassettes AHC1x16 and AHC4x16, allowing the formation of 16 wells that are 7.5 x 7.5 mm in size and configured in a 2 x 8 pattern at 9 mm centers. Use 1 each for AHC1x16 and four each for AHC4x16. Silicone replacement gaskets allow the demarcation of a 2 x 8 configuration of wells at 9 mm centers on microarray substrate slides for nucleic acid and protein microarray applications. Gaskets should be manually press fit into the top of the cassette prior to use. Gaskets should be stored in distilled water between uses to maintain hydration and proper fit with the top of the cassette.	GAHC1X16
Silicone Replacement Gasket GAHC1X24	Arrayit Silicone Replacement Gasket GAHC1X24 for the Arrayit Multi-Well Hybridization Cassette AHC1x24, allowing the formation of 24 wells that are 7.5 x 6.5 mm size and configured in a 3 x 8 pattern at 9 mm centers. The Arrayit GAHC1X24 silicone replacement gasket is used to create 24 separate reaction areas on a microarray substrate slide for nucleic acid and protein microarray applications. Gaskets should be manually press fit into the top of the cassette prior to use. Gaskets should be stored in distilled water between uses to maintain hydration and proper fit with the top of the cassette.	GAHC1X24
Silicone Replacement Gasket GAHC4X16	Arrayit Silicone Replacement Gasket GAHC4X16 for the Arrayit Multi-Well Hybridization Cassette AHC1x16, 4 gaskets per set. The Arrayit GAHC4X16 silicone replacement gaskets for Arrayit hybridization cassette AHC4X16 provide 64 individual reaction areas in a 2 x 8 x 4 pattern on four glass slides (1 x 25 x 76 mm). Gaskets should be manually press fit into the top of the cassette prior to use. Gaskets should be stored in distilled water between uses to maintain hydration and proper fit with the top of the cassette.	GAHC4X16
Replacement Gasket GAHC4X24	Arrayit Silicone Replacement Gasket GAHC4X24 for the Arrayit Multi-Well Hybridization Cassette AHC4x24, 1 gasket each. The Arrayit GAHC4X24 silicone replacement gasket for Arrayit hybridization cassette AHC4X24 provides 96 individual reaction areas in a 3 x 8 x 4 pattern on four glass slides (1 x 25 x 76 mm). Gaskets should be manually press fit into the top of the cassette prior to use. Gaskets should be stored in distilled water between uses to maintain hydration and proper fit with the top of the cassette.	GAHC4X24
Silicone Replacement Gasket GAHC4X8S	Arrayit Silicone Replacement Gasket GAHC4X8S for the Arrayit Multi-Well Hybridization Cassette AHC4x8S, 4 gaskets each. The Arrayit GAHC4X8S silicone replacement gaskets for Arrayit hybridization cassette AHC4X8S provide 32 individual reaction areas in a 1 x 8 x 4 pattern on four glass slides (1 x 25 x 76 mm). Individual well capacity is 100-200 µl. The GAHC4X8S replacement gasket is used to create 32 separate reaction areas on four microarray substrate slides for nucleic acid and protein microarray applications. Gaskets should be manually press fit into the top of the cassette prior to use. Gaskets should be stored in distilled water between uses to maintain hydration and proper fit with the top of the cassette.	GAHC4X8S
Silicone Replacement Gasket GAHC4X8L	Arrayit Silicone Replacement Gasket GAHC4X8L for the Arrayit Multi-Well Hybridization Cassette AHC4x8L, 1 gasket each. The Arrayit GAHC4X8L silicone replacement gasket for Arrayit hybridization cassette AHC4X8L provides 32 individual reaction areas in a 1 x 8 x 4 pattern on four glass slides (1 x 25 x 76 mm). Individual well capacity is 150-300 µl. The GAHC4X8L replacement gasket is used to create 32 separate reaction areas on four microarray substrate slides for nucleic acid and protein microarray applications. Gaskets should be manually press fit into the top of the cassette prior to use. Gaskets should be stored in distilled water between uses to maintain hydration and proper fit with the top of the cassette.	GAHC4X8L

Warranty

Arrayit life sciences testing platforms are sold for research purposes only. Arrayit brand products have been scientifically developed and are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All Arrayit brand products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any performance issues should be reported to Arrayit immediately. Arrayit’s liability is limited to the replacement of the product, or a full refund. Any misuse of this product is the full responsibility of the user, and Arrayit makes no warranty or guarantee under such circumstances. Pricing may vary up to 30% due to costs associated with distribution, import taxes, duties, customs clearance and shipping.