Arrayit Microarray Stripping Buffer A (1L of 1X Solution) is part of a 3-buffer set that allows microarray customers to re-use microarrays multiple times following hybridization and scanning. Arrayit Microarray Stripping Buffer A is a high-stringency buffer that strips and removes fluorescent DNA strands from DNA microarrays, while preserving the integrity of the microarray strands attached to the glass substrate slide. Arrayit Microarray Stripping Buffers A-C empower customers to strip microarrays in less than 30 minutes, enabling 2-10X microarray re-use depending on the method of manufacturing and other factors. Arrayit MSA can be used with microarrays from Arrayit, Agilent, Affymetrix and other manufacturers and is provided as 1,000 ml (1 liter) of 1X solution, filtered and ready to use upon arrival.
Buffers and Solutions - Microarray Stripping Buffers for Stripping and Reuse of Fluorescent Hybridized Microarrays in Basic Research, Life Sciences and Microarray Testing Applications
Arrayit Microarray Stripping Buffers are offered as a 3-buffer set that allows microarray customers to re-use microarrays multiple times following hybridization and scanning. Arrayit Microarray Stripping Buffers A, B and C strip and remove fluorescent DNA strands from fluorescent DNA microarrays, while preserving microarray integrity, enabling microarrays to be re-used up to 10 times depending on the method of manufacturing. Arrayit Microarray Stripping Buffers are compatible with microarray from Arrayit, Agilent, Affymetrix and microarrays from other manufacturers, and are sold as 1,000 ml (1 liter) of pre-filtered and ready-to-use 1X solution.
Table of Contents
- Introduction
- Quality Control
- Product Description
- Short Protocol
- Complete Protocol
- Recommended Equipment and Reagents
- Technical Notes
- Technical Support
- Scientific Publications
- Ordering Information
- Warranty
Introduction
Congratulations on taking a big step towards improving the economies of scale, quality and speed of your genomics research. This booklet contains a complete set of protocols outlining the steps and principles needed to use Arrayit Microarray Stripping Buffers.
Quality Control
Arrayit assures the performance of this product line. The finest scientific research was used to develop this product line. Rigorous quality control monitoring on a lot-by-lot basis guarantees that the components conform to the highest industry standards.
Product Description
Arrayit Microarray Stripping Buffers A, B and C (1L of 1X Solution) are an advanced 3-buffer set that allows DNA microarray stripping and reuse up to 10 times. Arrayit Microarray Stripping Buffers can improve the affordability of DNA microarrays for research, life sciences and testing applications.
Users will appreciate the following features:
- Supports multiple microarray technologies
- Strips fluorescent molecules hybridized to DNA microarrays
- Leaves microarrays intact for subsequent rounds of hybridization
- Improves affordability by allowing 2-10 uses
- Three buffer system strips, washes and rinses
- Compatible with printing, ink-jetting and in situ synthesis
- Supports Arrayit, Agilent, Affymetrix and others
- Buffers are used at room temperature for convenience
- 1X buffers are filtered, premixed and ready to use
Figure 1. Microarray slide stripping. Arrayit High-Throughput Microarray Wash Station (Cat. HTW) can be used in conjunction with Arrayit Microarray Stripping Buffers A-C to provide buffer mixing during the stripping steps. Arrayit HTW capacity is 25 glass slides (1 x 25 x 76 mm) and 500 ml of each of the 1X solutions.
Figure 2. Microarray slide drying. Arrayit High-Speed Centrifuges (Cat. MHC110V and MHC220V) can be used in conjunction with Arrayit Microarray Stripping Buffers A-C to drying the glass slides (1 x 25 x 76 mm) after the stripping steps. The drying time is 5 seconds per slide.
Short Protocol (Steps 1-7)
1. Transfer glass substrate slide microarrays to a High-Throughput Wash Station.
2. Wash 10 min at room temperature in 500 ml of Microarray Stripping Buffer A.
3. Transfer substrate slide rack to a second High-Throughput Wash Station.
4. Wash 10 min at room temperature in 500 ml of Microarray Stripping Buffer B.
5. Transfer substrate slide rack to a third High-Throughput Wash Station.
6. Wash 1 min at room temperature in 500 ml of Microarray Stripping Buffer C.
7. Spin glass substrate slides dry for 5 sec using Microarray High-Speed Centrifuge.
Complete Protocol (Steps 1-7)
1. Transfer glass substrate slide microarrays to a High-Throughput Wash Station. Arrayit High-Throughput Wash Stations Cat. HTW have a capacity for 25 glass microarray slides in the slide rack and 500 ml of 1X stripping buffer in the reservoir. Add 500 ml of Arrayit Microarray Stripping Buffer A to the HTW reservoir before adding the microarray slides. Make sure to keep the microarray slides hydrated at all times during the stripping steps as drying may elevate background fluorescence.
2. Wash 10 min at room temperature in 500 ml of Microarray Stripping Buffer A. Use a magnetic stir plate and moderate mixing during the stripping steps. Make sure to keep the microarray slides hydrated at all times. Arrayit HTW accommodates microarrays from Arrayit, Agilent and other manufacturers. Affymetrix customers can used the microfluidic station in conjunction with Arrayit stripping buffers for the stripping steps.
3. Transfer substrate slide rack to a second High-Throughput Wash Station. Quickly transfer the slides from the HTW containing 500 ml of 1X Arrayit Microarray Stripping Buffer A to a second HTW containing 500 ml of 1X Arrayit Microarray Stripping Buffer B.
4. Wash 10 min at room temperature in 500 ml of Microarray Stripping Buffer B. Moderate agitation on a magnetic stir plate should be used during this stripping step. Make sure to keep the microarray slides hydrated at all times. Arrayit HTW accommodates microarrays from Arrayit, Agilent and other manufacturers. Affymetrix customers can used the microfluidic station in conjunction with Arrayit stripping buffers for the stripping steps.
5. Transfer substrate slide rack to a third High-Throughput Wash Station. Quickly transfer the slides from the HTW containing 500 ml of 1X Arrayit Microarray Stripping Buffer B to a third HTW containing 500 ml of 1X Arrayit Microarray Stripping Buffer C.
6. Wash 1 min at room temperature in 500 ml of Microarray Stripping Buffer C. Moderate agitation on a magnetic stir plate should be used during this stripping step. Make sure to keep the microarray slides hydrated at all times. Arrayit HTW accommodates microarrays from Arrayit, Agilent and other manufacturers. Affymetrix customers can used the microfluidic station in conjunction with Arrayit stripping buffers for the stripping steps.
7. Spin glass substrate slides dry for 5 sec using Microarray High-Speed Centrifuge. Arrayit High-Speed Centrifuges Cat. MHC110V provide a rapid and convenient way to dry microarray slides after stripping. Make sure to insert the slide completely into the rotor and wear safety glasses at all times during use. A 5 second drying step should be sufficient to dry the microarray slide in preparation for the next round of hybridization.
Recommended Equipment and Reagents
Arrayit printing technology
High Throughput Wash Stations
Micro Spotting Solution Plus
Hybridization Cassettes
High-Speed Centrifuges
Microarray Slides
Technical Notes
- Designed to remove fluorescent probe molecules from microarrays post-hybridization
- Compatible with all glass-based microarrays including Arrayit, Agilent, Affymetrix and others
- The same incubation times should be used for microarrays enclosed in cassettes and cartridges
- Do not incubate >10 min or at elevated temperatures as this will impair microarray quality