Arrayit replacement gaskets for Microarray Hybridization Submersible Cassette RC1X24 featuring durable elastomer composition, gaskets are identical and interchangeable for top and lid, two gaskets per set.
Tools - Hybridization Cassettes - Microarray Hybridization Submersible Cassettes RC1X24 Provide Waterproof Hybridization Chambers for 24-Well Glass Substrate Slide Microarray Experimentation and Testing
Arrayit offers an affordable and innovative cassette to empower multi-well microarray testing in a 3 x 8 well pattern using Arrayit SuperMask 24 Microarray Substrate Slides (1 x 25 x 76 mm), which contain hydrophobic masks for well creation in a variety of surface chemistries. The submersible hybridization cassette allows temperature-controlled reactions without sample evaporation. Twenty four microarrays in a 3 x 8 pattern can be printed, reacted, scanned and quantified on a single glass substrate slide (1 x 25 x 76 mm), offering highly affordable sample multiplexing for research, life sciences and microarray testing.
Product Description
Designed for 24 microarray samples on a single microarray substrate slide (1 x 25 x 76 mm). The RC1X24 cassette is water-tight to prevent evaporation and empower submersion-based hybridization reactions including both short and extended reactions from 0-100°C using a temperature controlled water bath or oven. This 24 well submersible hybridization cassette fits one 24-well Arrayit SuperMask 24 Substrate Slide for 24 separate microarray reactions using a single cassette. No cover slips are required.
This submersible cassette is features durable, flat, precision-machined corrosion resistant alloy and high quality black elastomer gaskets to ensure proper alignment and strong well-to-well performance.
Specifications
- Arrayit Microarray Hybridization Submersible Cassette RC1X24 is designed for hybridization reactions featuring all types of microarrays including DNA, protein, peptide and others
- RC1x24 is designed for use with Arrayit SuperMask 24 Microarray Substrate Slides
- Multiplexed 24-well format in a 3 x 8 well pattern matches the SuperMask 24 well pattern
- Each well is 6 x 7 mm to accommodate up to 1,000 printed microarray elements
- Wells are set at 9.0 mm center-to-center spacing to match standard multichannel pipettes
- SuperMask 24 is highly compatible with Arrayit Stealth, 946 and PRO microarray printing technology
- SuperMask 24 is highly compatible with Arrayit SpotBot® and NanoPrint™ microarray printers
- RC1X24 Cassette reaction volumes are 35-75 µl per well
- RC1X24 Gaskets can be reused many times and are chemically inert
- RC1X24 Cassettes can be stacked in water baths and incubators to conserve space and ensure uniform reaction temperatures
- RC1X24 Cassettes support a wide range of reaction temperatures from 0-100°C
- Slots in cassette base facilitate microarray slide removal are use
- Rugged design ensures durability in your laboratory
- Inert cassette alloys and silicone gaskets provide pristine reaction conditions in each well
- Compact cassette size measures 2.7 x 6.1 x 11.2 cm
- Made of chemically resistant materials
- Durable gaskets can be reused many times
- Cut-away cassette bottom design allows visualization and rapid temperature equilibration
- Manual assembly and disassembly with no tooling required
- Locator pins in base and top ensure proper assembly
- Cost effective microarray testing through miniaturization and multiplexing
- Compatible with Arrayit's entire line of SuperMask 24 microarray slides. for a wide range of microarray applications.
Figure 2. SuperMask 24 Microarray Substrate Slides (1 x 25 x 76 mm) contain a teflon mask (black) that defines 24 wells in a 3 x 8 pattern. The gasket in the RC1X24 cassette top matches the hydrophobic mask on the slide to ensure well-to-well fidelity during the hybridization reactions. The mask also ensures that the microarray slide will release from the matching gasket easily after each use. Each well is 6 x 7 mm to accommodate 10-1,000 microarray spots. Rounded corners assure good coverage of reaction volume across all spots on the microarray. The mask does not interfere with microarray scans.
Use Recommendations
- Well volume is 35-75 µl. Do not exceed 75 µl as overfilling can result in cross contamination.
- After adding fluorescent probe mixtures to the wells, keep the cassette level to avoid contaminating the lid and reducing the volume of sample per well. In case of accidental cassette inversion, tap the assembled cassette firmly on a lab bench to re-locate the sample onto the slide.
- The reaction volume in each well of an assembled cassette can be viewed using the cut-out in the base. Remember to keep the cassette level during well inspection.
- RC1X24 reaction cassettes should be rinsed with distilled water and dried with polyester cleanroom wipes (Cat. MCW). For more intensive cleaning, RC1X24 cassettes may be sonicated for 5 min in 1% micro cleaning solution (Cat. MCS).
- After removing the lid, the assembled cassette top and base can be submerged in wash buffer for slide processing.
Gasket Information
Arrayit RC1X24 cassette gaskets exhibit good resistance to all standard buffers used in DNA, protein and other types of microarrays. Additionally the gasket is resistant to oils, UV and aging. Temperature range of the gasket material is -20°C to 110‚C. Gaskets are resistant to compression, tearing, chipping and to mild solvents, acids and bases. Strong solvents and acids and bases should be avoided.
SuperMask 24 Microarray Substrate Slides Click Here
Arrayit Microarray Multiplexing Benefits
1. Reduced cost and labor. Arrayit microarrays can multiplex, miniaturize and parallelize many assay types. Microarrays have the ability to multiplex and miniaturize to a much greater degree than other technologies. Arrayit's contact printing microarray platform can simultaneously capture data from single analytes to tens of thousands in a single binding reaction. Microarray scanners that support the standard glass slide format (1 x 25 x 76 mm) are installed in laboratories worldwide.
2. Favorable reaction kinetics and miniaturization reduce assay times and samples and reagent requirements. Every spot in the microarray is subjected to the same reaction and processing conditions for high precision and consistent data among technical replicates. Small spots mean less analyte and detection reagent, which reduces cost and makes microarray analysis less expensive than many other platforms.
3. Planar reaction kinetics give faster, more reproducible results. Arrayit printed microarrays feature capture reagents covalently bound to the surface of the microarray and therefore cannot interact with each other in solution as with solution-based assays. Arrayit microarrays feature high concentrations of target and probe molecules, which means faster binding kinetics, increased sensitivity and results within a few minutes or hours. A properly executed microarray assay will provide a more sensitive and reproducible results than many other platforms.
4. Focused, flexible mutiplexing in the range of 1-100,000 analytes meets the needs of a wide variety of applications. Arrayit microarrays can be read using high speed scanners. Microarrays can use diverse sample types including serum, amplified DNA, small molecules, carbohydrates, oligosaccarides, hybridomas, phage and others as capture analytes.
5. Standard glass slide format (1 x 25 x 76 mmm) open architecture allows market leading companies to provide supporting products. Our printed microarrays can be read using advanced microarray detection instruments and printed using microarray manufacturing instruments. Microarray detection is routinely performed using fluorescent, colorimetric and chemiluminescent detection to provide a flexible and open operating environment. Both CCD and laser/PMT architectures are available based on the end-users need for quantitation, cost and sensitivity.
Figure 3. Arrayit RC1X24 Hybridization Submersible Cassette fully assembled including the base, top, lid, internal gaskets and SuperMask 24 microarray substrate slide (1 x 25 x 76 mm). The lid is secured to the top using four thumbscrews tightened in a clockwise manner. The top is secured to the base using an additional four thumb screws tightened clockwise. The RC1X24 cassette is fully water-tight for 24-well multiplexing and temperature control in a water bath.
Figure 4. Arrayit RC1X24 Hybridization Submersible Cassette with the lid removed showing the assembled base, top, internal gaskets and SuperMask 24 microarray substrate slide (1 x 25 x 76 mm). The 24-wells allow 24-sample multiplexing on a single slide. Wells are set at 9.0 mm centers in a 3 x 8 pattern as shown.
Figure 5. Arrayit RC1X24 Hybridization Submersible Cassette lid in inverted orientation that seals each of the 3 x 8 (24 wells) of the cassette using a tight fit and an elastomer gasket. The RC1X24 lid assembles to the top of the cassette using four thumb screws tightened clockwise. The lid prevents sample evaporation during the hybridization reaction and allows submersion for temperature control in a water bath.
Figure 6. Arrayit RC1X24 Hybridization Submersible Cassette shown in inverted orientation and fully assembled including the base, top, lid, internal gaskets and SuperMask 24 microarray substrate slide (1 x 25 x 76 mm). The SuperMask 24 wells in a 3 x 8 pattern are sealed with a gasket fitted into the top of the cassette. Arrayit RC1X24 empowers DNA and protein microarray multiplexing in a convenient, water-tight cassette for research, life sciences and microarray testing applications.
Figure 7. Arrayit RC1X24 Hybridization Submersible Cassette with the assembled base, top, internal gaskets, SuperMask 24 microarray substrate slide (1 x 25 x 76 mm) and the lid removed. The RC1X24 cassette wells are spaced at 9.0 mm centers across the cassette, allowing users to pipette samples into the 24 wells using manual and automated pipettes.
Figure 8. Arrayit RC1X24 Hybridization Submersible Cassette with the assembled base, top, internal gaskets, SuperMask 24 microarray substrate slide (1 x 25 x 76 mm) and the lid removed. The RC1X24 cassette wells are spaced at 9.0 mm centers down the cassette, allowing users to pipette samples into the 24 wells using manual and automated pipettes.
Figure 9. Arrayit RC1X24 Hybridization Submersible Cassette base with a SuperMask 24 microarray substrate slide (1 x 25 x 76 mm) seated properly with the corner chamfer facing upper right. The SuperMask 24 defines 24 wells (6 x 7 mm) in a 3 x 8 pattern and each of the 24 wells is spaced at 9.0 mm centers, allowing users to pipette samples into the 24 wells using manual and automated pipettes. Two locator pins in the base locate to two apertures in the top, ensuring that the cassette is assembled properly each time for unambiguous sample tracking.