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Custom Peptide Synthesis and Services for Microarrays

Price: $98.00
Item Number: CPS
Arrayit offers the finest custom peptide synthesis and microarray processing services available including highly competitive per-residue pricing on a complete line of peptides for microarray manufacturing including amino linkers, other modifications, in 0.5 mg or 2.0 mg synthesis scales, and various purification strategies. Peptides up to 20 residues are synthesized and provided unpurified or purified and lyophilized in single tubes in a 96-well microplate format. Arrayit utilizes the most advanced peptide synthesis chemistries for the highest purity available for strong hybridization signal strengths, low background and highly durable chemical linkers and fluorescent dyes for epitope mapping, functional domain analysis, protein expression analysis and all other microarray, life sciences and research applications. Arrayit also offers complete peptide microarray services including peptide microarray manufacturing, blocking, reactions, washing, rinsing, scanning and data analysis. Pricing shown is for a low density peptide microarray printed in volume. Please contact us for exact pricing information and lead time for your project.

Services - Peptide Synthesis, Microarray Manufacturing and Processing Services for Structural Motif, Linear Epitope Analysis and Antigen and Auto-Antibody Binding

Arrayit Custom Peptides and Peptide Microarray Manufacturing Services provide unprecedented access to high quality peptides and peptide microarrays at affordable prices. Peptides and peptide microarrays are manufactured using the most advanced technology available including cleanroom environments, linear drive robotics, atomically smooth glass substrate slides and superior coupling chemistries. Our peptides and peptide microarrays are widely applicable to research and clinical applications in the life sciences and molecular diagnostics.

Arrayit Peptide Microarray FAQs
Microarrays are compatible with all standard slide based fluorescent microarray scanners, quantification software and processing tools. Microarray are manufacturing using Arrayit's patented technology, US Patent 6,101,946. Thousands of microarrays can be manufactured with a single peptide library synthesis. Arrayit can provide technical assistance and all tools necessary for processing, scanning and quantifying the microarrays. We can also empower end users to manufacture their own peptide microarrays using the SpotBot and NanoPrint Microarrayers, as well as provide complete peptide microarray services using customer peptide sequences and samples.

Peptides are covalently bound to a glass slide microarray substrate and available for a wide variety of binding assays including:

  • biomarker discovery
  • antibody profiling
  • epitope mapping
  • immunoassays
  • protein interactions
  • kinase screening
  • protease screening
  • phosphatase screening
  • affinity profiling
  • protein fingerprinting

Arrayit Peptide Microarrays are stable for up to 1 year at room temperature.

Peptide sequences on scan be as long as 20 amino acids.

Arrayit's patented microarray manufacturing technology empowers from 10 to tens of thousands of peptides per microarray with extremely low CV’s. Multiple microarrays per slide substrate can also be provided.

Ex-Situ 100% mass spec QC on each peptide before microarrays are manufactured.

Microarray format provides very low reaction volumes, from 10 to 500 micro-liters.

Peptide Microarrays can be manufactured with any number of technical replicates and controls.

Peptide microarrays are compatible with all standard slide based microarray scanners. For best results consider using the Innoscan 710 Microarray Scanner.

Compatible with standard ELISA protocols, formats can be provided to be compatible with ELISA processing automation. see:

peptide microarrays
Figure 1. Shows that the FLAG peptides epitopes were recognized by the ANTI-FLAG antibodies, but not by the ANTI-CD28 antibody used as an isotype matched control (negative control). In addition, the scrambled FLAG sequence that was used as another negative control was also not recognized by the ANTI-FLAG antibodies.  These results indicate the specificity of the peptide-antibody binding.  Figure 1 also shows that peptides containing additional sequences from both PILL and GST demonstrated enhanced reactivity with the ANTI-FLAG antibody.  Adding PILL or GST sequences to the peptides did not enhance reactivity to the irrelevant antibody. Data courtesy of Dr. Jonathan Graf and Dr. John Imboden, UCSF. Peptides courtesy of Sigma-Aldrich. Peptides can be up to 20 amino acids in length.

Figure 2. A overlapping series of peptides corresponding to a linear protein sequence can be used to identify the eptitope of a primary monoclonal antibody.

Figure 3. Peptide microarrays can be used to identify antibodies and other binding proteins in human serum and other customer samples. Please contact us ( for complete peptide microarray services pricing including manufacturing, reactions, scanning and data analysis.

Arrayit’s peptide microarray capabilities are well documented, e-mail us for a list of publications Please also see google scholar.

Applications of Peptide Microarrays
Identification of protein-protein interaction sites
Identifying protein-protein interaction sites is an extremely valuable tool enabling the design inhibitors, enhancers as well as providing information on binding regions of proteins.

Epitope Mapping
Epitope mapping identifies antibody binding domains. These regions can be used in diagnostics or even implemented in vaccine for drug development.

Drug development
Drug developers want a molecule that specifically interacts with a target protein. Molecules designed to compete with or crowd out other proteins which would otherwise bind to the target, result in either blocking or enhancing protein function.

Diagnostics & Identification of Biomarkers
Peptide microarrays can diagnose disease. Peptides as biomarkers in a miniaturized and multiplexed format test can simultaneously screen for dozens to thousands of biomarkers in a single assay with an eaiser and less expensive protocol than DNA microarrays.

Figure 4.
Peptide microarray formats include 1, 2, 4, 8 and 24 individual microarrays per substrate slide (1 x 25 x 76 mm). These formats are compatible with our gaskets and microarray hybridization cassettes. Click here to view tools.

Arrayit life sciences products are sold for research purposes only. Arrayit brand products have been scientifically developed and are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All Arrayit brand products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any performance issues should be reported to Arrayit immediately. Arrayit’s liability is limited to the replacement of the product, or a full refund. Any misuse of this product is the full responsibility of the user, and Arrayit makes no warranty or guarantee under such circumstances. Pricing may vary up to 30% due to costs associated with distribution, import taxes, duties, customs clearance and shipping.

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