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Blocking Buffer for SuperProtein Substrates, 250 ml of 1X solution
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Item Number: SPBB
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Arrayit SuperProtein Blocking Buffer is designed for blocking the portions of microarrays that can bind proteins after printing to eliminate background following the incubation or hybridization reaction of protein microarrays. Users will appreciate the following features: Ultra-pure reagents used for buffer formulation, purified by sterile filtration, provided as 1X ready to use concentrate, recommended for use on SuperProtein Substrates, but compatible with other protein membrane based microarray surface chemistries, easy coverslip blocking procedure, 250 ml volume provided efficiently blocks thousands of microarrays, will not react with proteins and DNA. Arrayit BlockIt Microarray Blocking Buffer is formulated for blocking the portions of microarrays that are still reactive after printing to eliminate fluorescent background following the incubation or hybridization reactions of protein and DNA microarrays. Ultra-pure reagents used for buffer formulatiom are purified by sterile filtration and provided as ready-to-use 1X solution, BKT is recommended for SuperEpoxy slides and is compatible with other DNA and protein microarray slides including SuperNitro. Easy coverslip or tray-based blocking procedures efficiently blocks hundreds of microarrays. Ships overnight on wet ice as 250 ml of 1X buffer.
Microarray Buffers - Blocking Solutions - SuperProtein Blocking Buffer to Enhance Signal Strength and Reduce Non-Specific Binding on Nitrocellulose Microarrays
Designed to block SuperProtein, SuperPVDF, SuperNitro membrane based microarray surface chemistry prior to protein binding reactions for ultra low colorimetric background. For glass based protein microarrays use BlockIt. For assays sensitive to low amounts of detergent use SuperProtein Blocking Buffer.
Table of Contents
- Introduction
- Quality Control
- Product Description
- Technical Assistance
- Short Protocol
- Complete Protocol
- Troubleshooting Tips
- Recommended Requirements
- Ordering Information
- Warranty
Introduction
Congratulations on taking a big step towards improving the economies of scale, quality and speed of your proteomics research. This booklet contains a complete set of protocols outlining the steps and principles needed to use the Arrayit brand blocking buffer for membrane-based surface chemistry. This product is design for use prior to incubation and hybridization reactions involving protein and DNA microarrays.
Quality Control
Arrayit assures the performance of these products, the finest scientific research went into their development. Store at 4 Degrees Celsius, the product has a 1-year shelf life when handled and stored properly.
Product Description
The buffer is designed for blocking the portions of microarrays that can bind proteins after printing to eliminate background following the incubation or hybridization reaction of protein microarrays. Users will appreciate the following features:
- Ultra-pure reagents used for buffer formulation
- Purified by sterile filtration
- Provided as 1X ready to use concentrate
- Recommended for use on SuperProtein Substrates, but compatible with other protein membrane based microarray surface chemistries
- Easy coverslip blocking procedure
- 250 ml volume provided efficiently blocks thousands of microarrays
- Will not react with proteins and DNA
Technical Assistance
Please contact us if you have any comments, suggestions, or if you need technical assistance. By electronic mail: arrayit@arrayit.com (under the subject heading, please type Arrayit technical assistance). By email: arrayit@arrayit.com, Monday–Friday PST 8:00am - 8:00pm. Please remember that we want to hear about your successes!
Short Protocol for Protein Microarrays
Wash microarray with PBS and then incubate microarray in 1X blocking buffer under a coverslip for 1 hour prior to protein binding reactions.
Complete Protocol for Protein Microarrays
Protein binding to our membrane surfaces is stable and the microarrays can be washed, blocked and reacted without sufficient loss of coupled protein. Block the printed microarray surface using a 1-hour incubation at room temperature in 1X SuperProtein Blocking buffer. Perform this reaction under a coverslip using 4 ul of buffer for every cm2 of coverslip area. Once the printing process is complete, wash the printed microarrays 2 times for 15 minutes each in a High Throughput Wash Station in 1X PBS to remove unbound protein molecules and buffer components from the SuperProtein Substrates.A Hybridization Cassette can be used to keep the blocking reaction from drying out. SuperProtein blocking buffer will couple to un-reacted binding sites of the surface and prevent unspecific binding and background. After blocking, wash the microarrays to remove excess buffer. Washing three times for 1 min each at room temperature with 1X PBS in a High Throughput Wash Station. DO NOT USE DETERGENT BASED buffers on the SuperProtein nor SuperPVDF, low concentrations are compatible with SuperNitro.
Troubleshooting Tips
If microarray shows high background:
- Confirm that the intrinsic background of the microarray is low to start with.
- Be sure to not let any sample dry on the microarray surface during the binding reaction.
- Make sure to purify away unincorporated components of any labeling reactions.
- Take care to block for the recommended time (1 hour).
- Pay attention to the proper handling and shelf life of the buffer (1 year shelf life, Store at 4 C).
- Do not skip washing the microarray after the blocking step.
- If colorimetric labeling was used, be sure to optimize development times
Recommended Products
Ordering Information
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Product
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Description
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Catalog
ID
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BlockIt™ Blocking Solution
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Arrayit BlockIt™ Blocking
Solution is an advanced microarray blocking solution designed to inactivate
reactive groups remaining post-printing on SuperEpoxy, SuperAmine, SuperAldehyde
and other glass microarray substrate slide surfaces. BlockIt™ Blocking
Solution greatly reduces background noise while maintaining full signal
intensities for a wide spectrum of DNA and protein microarray applications.
Ultra-high purity blocking buffer for coverslip and solution-based blocking
of protein and DNA microarrays, 250 ml of 1X buffer, ships on cold packs.
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BKT
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BlockIt™ HT Blocking
Solution
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Arrayit BlockIt™ HT
Blocking Solution is an advanced microarray blocking solution designed to inactivate
reactive groups remaining post-printing on SuperEpoxy, SuperAmine,
SuperAldehyde and other glass microarray substrate slide surfaces. BlockIt™
Blocking Solution greatly reduces background noise while maintaining full
signal intensities for a wide spectrum of DNA and protein microarray
applications. High purity blocking buffer for bulk solution high-throughput
(HT) blocking of protein and DNA microarrays for use with the High Throughput
Wash Station, 1000 ml of 1X buffer, ships on cold packs.
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BKTHT
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BlockIt™ Plus Blocking
Buffer
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Arrayit BlockIt™ Plus
Blocking Solution often works when BlockIt™ is shown to be less efficient
than required. Greatly reduces background noise while maintaining full signal
intensities for a wide spectrum of DNA and protein microarray applications.
Ultra-high purity blocking buffer (translucent), for blocking of protein and
DNA microarrays, 250 ml of 1X buffer, ships on cold packs.
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BKTP
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Microarray Blocking Buffer
Plus Automation Formulation
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Arrayit Microarray
BlockIt™ Plus Blocking Solution Automation Formulation should be used in
cases where a greatly reduced background is required, while maintaining full
signal intensities for a wide spectrum of DNA and protein microarray
applications particularly those utilizing workstations, liquid handling
robots, multiplex hybridization cassettes and other high-throughput hardware
and automation equipment. 1L of 1X buffer arrives pre-mixed and ready to use,
ships on cold packs.
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BKTPL
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SuperProtein Substrate
Blocking Buffer
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Arrayit SuperProtein
Substrate Blocking Buffer is an advanced microarray blocking solution
designed to inactivate reactive groups remaining post-printing on our
membrane-based microarray surfaces such as nitrocellulose, PVDF and nylon.
SuperProtein Blocking Solution greatly reduces background noise while
maintaining full signal intensities for a wide spectrum of protein microarray
applications. 250 ml of 1X buffer, ships on cold packs.
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SPBB
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SuperStreptavidin
Microarray Blocking Buffer
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Arrayit SuperStreptavidin Microarray
Blocking Buffer is an advanced protein and small molecule blocking buffer
that blocks and inactivates SuperStreptavidin microarray substrate slides
prior to staining, greatly reduces background for all applications, 250 ml of
1X buffer solution, ships on cold packs.
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SBB
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SuperAvidin Blocking
Buffer
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Arrayit SuperAvidin
Blocking Buffer is an advanced protein and small molecule blocking buffer
that blocks and inactivates SuperAvidin microarray substrate slides prior to
staining, greatly reduces background for all applications, 250 ml of 1X
buffer solution, ships on cold packs.
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ABB
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ChemBlock™
Microarray Chemical Blocking Buffer
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Arrayit ChemBlock™
Microarray Blocking Buffer features a proprietary mixture of chemical
blocking agents, buffering components and detergents to inactive aldehyde and
epoxide reactive groups after microarray printing and before DNA and protein
microarray reactions to eliminate background binding observed with
traditional protein blocking buffers. ChemBlock™ is highly recommended for
serum reactions and other protein microarray applications requiring uniform
and extremely low background signals across a large number of blood
specimens, 0.2 µm filtered, store at -20°C, 1 liter of 1X solution, ships on
cold packs.
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CHE
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Warranty
Arrayit life sciences
products are sold for research purposes only. Arrayit brand products have been
scientifically developed and are sold for research purposes. Extreme care and
exact attention should be practiced in the use of the materials described herein.
All Arrayit brand products are subject to extensive quality control and are
guaranteed to perform as described when used properly. Any performance issues
should be reported to Arrayit immediately. Arrayit’s liability is limited to
the replacement of the product, or a full refund. Any misuse of this product is
the full responsibility of the user, and Arrayit makes no warranty or guarantee
under such circumstances. Pricing may vary up to 30% due to costs associated
with distribution, import taxes, duties, customs clearance and shipping.
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