Arrayit Blood Card DNA Isolation Kit

Microarray Testing - Arrayit Blood Cards, Private Label Blood Cards, Blood Card Kits and DNA and Serum Isolation Kits

Arrayit has developed an advanced blood card serum separation technology that collects and fractionates whole blood into red blood cells, white blood cells and serum components quickly, easily and affordably. Our unique linear separation chromatography process collects 5 droplets of whole blood and separates and dries the whole blood components in 10 minutes. Arrayit Blood Cards can be used to fractionate, ship, sample and store samples for a variety of applications in research, veterinary medicine and pathology including protein-based allergy testing and cancer research. Arrayit Blood Cards can also be used to isolate DNA and mRNA samples for genotyping and expression profiling applications. Private labeling is available for large original equipment manufacturer (OEM) accounts.

Table of Contents

• Introduction

• Quality Control

• Product Description

• Product Contents

• Whole Blood Contents

• Product Protocols

• Technical Assistance

• Troubleshooting Tips

• Recommended Products

• Scientific Publications

• Ordering Information

• Warranty

Introduction

Arrayit has developed an advanced new blood card serum separation technology for diverse applications in research, veterinary testing and human in vitro microarray testing.

Quality Control

Arrayit Blood Cards are manufactured using the highest level of quality control (QC) and quality assurance (QA) available. Quality control and quality assurance of each manufactured lot ensures that our Blood Card performance will meet or exceed the highest research, veterinary, pathology and microarray testing standards.

Product Description

Arrayit Blood Card serum separation technology offers an advanced card-based whole blood collection method that collects, separates and stores whole blood components for research, veterinary and microarray testing. Users will appreciate the following features of this product:

• Market’s first high-performance blood card technology

• Based on advanced competitive linear flow chromatography

• Separates whole blood components in less than 5 minutes

• Collection, fractionation, storage and shipping in a single product

• Highly purified serum samples for protein microarrays

• Reduces background and enhances signal strength in antibody assays

• Excellent for biomedical research and pathology applications

• Ideal for veterinary applications including allergy testing

• 5 drop whole blood collection capacity

• Compatible with both finger stick and intravenous whole blood samples

• 6 mm sample disc yields 1-2 µg serum protein

• Supports DNA testing including VIP genotyping assays

• Supports mRNA testing including gene expression profiling assays

• Unique patient identification code ensures proper sample identification

• Conventional one-dimensional barcoding re-confirms sample tracking

• Two-dimensional Quick Response Code (QR Code) permits mobile device web access

• Compact 2 x 3.5” (5 x 8.75 cm) “business card” physical dimensions

• Extremely lightweight at 1.3 g per card

• Customer Reference Card provides permanent record and easy-to-follow instructions

• Compatible with microarrays and other high-throughput technologies

• Easily automated into 96-well sample disc formats

• Manufactured and packaged in a cleanroom environment

• Free of biological and chemical contaminants

• Ultra-thin proprietary membrane speeds fractionation and improves sample integrity

• Suitable for parentage and forensic testing applications

• Reduces sample shipping costs and conserves laboratory space

• 10-year DNA and protein stability

Figure 1. Arrayit Private Label Blood Cards (Cat. ABCP) and Blood Card Kits with logo, user instructions, company address and custom QR codes are available for large OEM customers. Arrayit Blood Card technology is for research use only.

Blood Card Kit Contents

• Blood Card, 1 each

• Customer Reference Card, 1 each

• Fingerstick lancets, 2 each

• Silver anti-static shipping envelope, 1 each

Whole Blood Contents

Arrayit Blood Cards collect 5 droplets of human blood, which contains the following cellular and molecular components:

• 100 µl of whole human blood

• 4.8 x 10^-8 red blood cells

• 7.3 x 10^-5 white blood cells

• 40 µl of serum

• 2.9 mg total serum protein

• 250 µg total serum protein (6 mm disc)

• 2.5 µg white blood cell total RNA (6 mm disc)

• 0.5 µg total genomic DNA (6 mm disc)

Serum Isolation Kit Contents (25 samples)

• Spin columns, 25 each

• Microfuge tubes (1.5 ml), 25 each

• Serum elution buffer (1X), 50 ml of 1X

DNA Isolation Kit Contents (25 samples)

• Spin columns, 25 each

• Microfuge tubes (1.5 ml), 75 each

• Microfuge wash tubes (1.5 ml), 25 each

• DNA Buffer A (1X), 15 ml

• DNA Buffer B (1X), 15 ml

• DNA Buffer C (1.33X), 15 ml

• DNA Buffer D (1X), 15 ml

• DNA Buffer E (1X), 15 ml

Figure 2. Arrayit Blood Card Kits (Cat. ABCK) include a Customer Reference Card as a permanent record of the sample. The Reference Card contains a Patient ID number that matches the Blood Card, a traditional barcode with serial number and a Quick Reference Code (QR Code) two-dimensional barcode for accessing the Blood Card webpage using smart phones, tablets and other mobile devices. The Customer Reference Card also includes space for a hand-written patient name and sampling date. The Reference Card has business card dimensions of 2 x 3.5” (5 x 8.9 cm) for convenient storage and referencing. The back of the card contains instructions for blood sampling (see Figure 3).

Figure 3. Arrayit Blood Card Kits (Cat. ABCK) include Blood Card Instructions as a 9-step blood sampling procedure on the back of the Customer Reference Card (see Figure 2 for front view). The Reference Card has business card dimensions of 2 x 3.5” (5 x 8.9 cm) for convenient storage and referencing. The front of the Reference Card contains a Patient ID number that matches the Blood Card, a traditional barcode with serial number, a Quick Reference Code (QR Code) two-dimensional barcode for accessing the Blood Card webpage using smart phones, tablets and other mobile devices, and a space for a hand-written patient name and sampling date (see Figure 2).

Figure 4. Arrayit Blood Card Kits (Cat. ABCK) contain a Blood Card, Customer Reference Card, two fingerstick lancets, and a silver anti-static envelop for shipping. Cut the plastic kit pouch with a scissors to remove the kit components and follow instructions carefully before, during and after use.

Figure 5. Arrayit Blood Card fingerstick lancet. Blood sampling is achieved by placing the middle finger on your left hand directly on the red, spring-loaded sampling platform as shown and pressing down firmly. Follow instructions carefully before, during and after use.

Figure 6. Arrayit Blood Card shown 10 minutes after placing 5 large drops of whole human blood inside the red box. The red blood cell components remain on the left portion of the linear transfer membrane and the serum (yellowish fraction) partitions to the right. Arrayit Blood Cards can be used for DNA, mRNA and protein analysis by taking sample discs of these respective fractions.

Blood Sampling (Short Protocol)

1. Wash your hands with soap and hot water for 1 minute and dry your hands with a lint-free wipe.

2. Open the plastic product envelop and place the kit components on the bench top.

3. Remove the plastic safety cap from the lancet.

4. Place the lancet on the bench top with the red sampling platform facing upward.

5. Place your left middle fingertip on the lancet sampling platform.

6. Press down firmly on the lancet.

7. Place 5 large drops of whole blood inside the red box on the Blood Card.

8. Allow the blood to separate on the Blood Card for 5 minutes.

9. Allow the Blood Card to dry for 5 minutes.

10. Place the Blood Card in the silver anti-static shipping envelop for storage and shipping.

Blood Sampling (Complete Protocol)

1. Wash your hands with soap and hot water for 1 minute and dry your hands with a lint-free wipe. This step is important because it removes bacteria and other contaminants from your hands prior to sampling. The use of hot water also improves blood circulation in your finger tips, which improves the efficiency of the sampling procedure. Make sure to wash for at least 1 minute as shorter wash periods will reduce blood flow. Please avoid topical cleaning with alcohol swabs and other solvents as this will dehydrate the sampling area and reduce blood flow. Make sure to dry your hands completely with a lint-free laboratory wipe to ensure dry finger tips prior to blood sampling.

2. Open the plastic product envelop and place the kit components on the bench top. Use a scissors or other cutting implement to carefully cut the top of the plastic envelop and then remove the Blood Card, Customer Reference Card, two lancets and the silver anti-static shipping envelope. The kit components (see Figure 4) should be placed on a smooth, clean and level surface such as a laboratory bench or clinical table.

3. Remove the plastic safety cap from the lancet. The pink lancets contain a clear plastic safety cap on the top of the lancet to protect the lancet from premature use. Remove the plastic cap carefully immediately prior to use.

4. Place the lancet on the laboratory bench with the red sampling platform facing upward. The lancet contains a red, spring-loaded sample platform. Make sure the sampling platform is facing upwards prior to sampling.

5. Place your left middle fingertip on the lancet sampling platform. Place the tip of your middle finger softly in the center of the sampling platform with your finger tip aligned directly in the center of the 3 mm orifice located in the center of the platform (see Figure 5). Because of the proximity to your heart, blood pressure and blood flow in the left hand is slightly greater than in your right hand, and the left middle finger is chosen for this reason.

6. Press down firmly on the lancet. Press down firmly on the lancet platform to initiate the sampling process. The lancet uses a spring-loaded stainless steel sampling device approximately 350 µm in diameter to create a nearly invisible sampling site on your left middle finger tip. The sampling process is fast and nearly undetectable.

7. Place 5 large drops of whole blood inside the red box on the Blood Card. For best results, make sure you are standing up for this step to increase blood pressure and blood flow. Use your non-sampling hand to squeeze your finger tip firmly and create five blood droplets. Place the droplets directly in the center of the red box of the Blood Card, making light contact between the droplet and the Blood Card membrane. Avoid touching the Blood Card membrane directly with your fingertip as this may interfere with the separation process. A properly lanced finger tip should produce 5 large drops or 100 µl of whole blood in less than one minute. If blood flow stops, it may be necessary to use the second lancet provided with the kit to obtain 5 large blood droplets.

8. Allow the blood to separate on the Blood Card for 5 minutes. After placing 5 large blood droplets in the center of the red box, allow 5 minutes for the blood components to separate on the Blood Card. The Blood Card should be placed level and flat on the laboratory bench during the separation process to permit even flow of the blood components. The blood components should separate from left to right on the card such that the red cell components remain to the left side of the separation membrane and the yellowish serum components migrate rightward during the 5 minute process. Five large blood droplets should produce a fractionation result similar to Figure 6. Customers using whole blood obtained by intravenous blood draws should place 5 drops or 100 µl of blood inside the red box.

9. Allow the Blood Card to dry for 5 minutes. Following the 5 minute fractionation process, incubate the Blood Card on the laboratory bench for an additional 5 minutes to allow drying of the fractionated whole blood components. The drying process enhances the stability of the blood components for sampling and storage and ensures that the components will remain firmly adhered to the Blood Card during shipping. Avoid making direct contact with the separated blood components at all times during the sampling, fractionation and drying steps as this can contaminate the samples and complicate downstream analysis.

10. Place the Blood Card in the silver anti-static shipping envelope for storage and mailing. Open the zip-lock located at the top of the silver anti-static shipping envelope and place the dried Blood Card into the envelope. Re-seal the zip-lock located at the top of the silver envelope and mail to the proper laboratory for testing. Alternatively, the Blood Card in the sealed silver anti-static envelope can be stored in a cool dark drawer or storage cabinet for future testing. Make certain to retain your Customer Reference Card as a permanent record of your sample, making sure to write your name and the sampling date on the card for future reference. The patient identification number located on your reference card is identical to the patient identification number on the Blood Card.

Figure 7. Arrayit Blood Card sample disc isolated 24 hours after whole blood collection. A 6 mm round disc containing fractionated serum was obtained using the Arrayit Blood Card Sample Disc Punch (Cat. ASDP). Serum discs of this diameter contain approximately 250 µg of total serum protein containing peptides, antigens, antibodies and other analytes of scientific, research, veterinary and pathology interest.

Figure 8. Arrayit Blood Card Sample Disc Punch (Cat. ASDP) enables precision manual generation of 6 mm sample discs for DNA, mRNA and serum samples. The disc punch is manufactured using injection molded plastic for resistance to light solvents. Spring loading allows easy manual operation. Sample discs are automatically released from the disc punch to allow easy transfer into microfuge tubes and microplates.

DNA Isolation (Short Protocol)

Note: Please wear nitrile gloves and safety glasses at all times during this procedure.

1. Obtain a 6 mm sample disc from the blood card.

2. Place the sample disc in a 1.5 ml microfuge tube.

3. Add 200 µl DNA Buffer A and 100 µl DNA Buffer B to the microfuge tube.

4. Vortex the microfuge tube vigorously for 20 sec and incubate 10 min.

5. Centrifuge the microfuge tube at 14,000 x g for 2 min.

6. Transfer the 100 µl aqueous (top) fraction to a fresh 1.5 ml microfuge tube.

7. Re-extract the sample disc two times with 50 µl DNA Buffer B.

8. Combine the three aqueous (top) fractions to create a 200 µl volume of DNA sample.

9. Add 200 µl DNA Buffer A to the 200 µl aqueous DNA sample.

10. Vortex the microfuge tube vigorously for 20 sec.

11. Centrifuge the microfuge tube at 18,000 x g for 2 min.

12. Transfer the 200 µl aqueous (top) fraction to a fresh 1.5 ml microfuge tube.

13. Add 600 µl of DNA Buffer C and vortex for 10 sec.

14. Transfer the 800 µl DNA sample to a spin column seated in a microfuge wash tube.

15. Centrifuge the spin column assembly at 1,000 rpm for 10 sec and discard the eluent.

16. Add 125 µl of DNA Buffer D to the spin column.

17. Centrifuge the spin column assembly at 1,000 rpm for 10 sec and discard the eluent.

18. Repeat wash steps 16 and 17 twice more.

19. Centrifuge the spin column assembly at 1,000 rpm for 60 sec to dry the spin column.

20. Add 50 µl of DNA Buffer E directly onto the spin column membrane.

21. Incubate the spin column for 5 min to resolubilize the DNA.

22. Centrifuge the spin column assembly at 1,000 rpm for 60 sec to elute the DNA.

23. Dry the DNA sample by vacuum centrifugation for downstream use.

DNA Isolation (Complete Protocol)

Note: Please wear nitrile gloves and safety glasses at all times during this procedure.

1. Obtain a 6 mm sample disc from the blood card. Use an Arrayit Sample Disc Punch to obtain a 6 mm round sample disc from the cellular “red” portion of the blood card. The sample disc will include white blood cells that contain nuclear and mitochondrial DNA. The plunger of the Disc Punch should be cleaned with distilled water and 100% ethanol after each use to prevent cross-contamination.

2. Place the disc in a 1.5 ml microfuge tube. Use clean forceps to carefully transfer the sample disc to a 1.5 ml microfuge tube.

3. Add 200 µl DNA Buffer A and 100 µl DNA Buffer B to the microfuge tube. Pipette 200 µl of DNA Buffer A and 100 µl of DNA Buffer B into the 1.5 ml microfuge tube. These buffers disrupt blood cells, inactivate cellular DNases that can degrade DNA and solubilize the DNA into the aqueous phase.

4. Vortex the microfuge tube vigorously for 20 sec and incubate 10 min. Close the 1.5 ml microfuge tube cap and vortex the tube vigorously for 20 seconds to disrupt the cells and elute the DNA from the blood card membrane. The 10 minute incubation at room temperature will ensure complete cellular disruption and efficient DNA recovery.

5. Centrifuge the microfuge tube at 14,000 rpm for 2 min. Transfer the 1.5 ml microfuge tube to a microcentrifuge and spin the tube at the full speed of 14,000 revolutions per minute (rpm) or 18,000 x g for 2 minutes to separate the phases. The blood card sample disc and insoluble material partitions in the bottom layer and the DNA partitions in the soluble aqueous (top) layer. The bottom layer should appear dark red and the top layer should appear clear to lightly beige in color.

6. Transfer the 100 µl aqueous (top) fraction to a fresh 1.5 ml microfuge tube. Use a 200 µl pipette tip with a barrier plug to transfer the 100 µl top layer containing the DNA to a fresh 1.5 ml microfuge tube. Pipette the top layer slowly and carefully to avoid transferring the bottom layer or interface.

7. Re-extract the sample disc two times with 50 µl DNA Buffer B. After transferring the 100 µl top layer containing the DNA, add 50 µl of fresh DNA Buffer B to the bottom layer in the 1.5 ml microfuge tube and vortex the tube vigorously for 20 seconds to disrupt additional cells and solubilize additional DNA. Centrifuge the 1.5 ml microfuge tube at 14,000 rpm for 2 minutes to separate the phases, and transfer the 50 µl top layer to the microfuge tube containing the 100 µl DNA sample. Repeat this process once more and transfer the 50 µl top layer to the microfuge tube containing the 150 µl DNA sample.

8. Combine the three aqueous (top) fractions to create a 200 µl volume of DNA sample. After the three extraction cycles, the 1.5 ml microfuge tube should contain a total volume of 200 µl containing the DNA sample. The DNA sample should appear clear to lightly beige in color.

9. Add 200 µl DNA Buffer A to the 200 µl aqueous DNA sample. To the 1.5 ml microfuge tube containing the 200 µl DNA sample, add 200 µl of DNA Buffer A using a 1 ml pipette tip.

10. Vortex the microfuge tube vigorously for 20 sec. Obtain the 1.5 ml microfuge tube containing 200 µl of DNA sample and 200 µl of DNA Buffer A and vortex the tube vigorously for 20 seconds to mix the phases and remove any remaining insoluble material from the DNA sample.

11. Centrifuge the microfuge tube at 18,000 x g for 2 min. Transfer the 1.5 ml microfuge tube containing 200 µl of DNA sample and 200 µl of DNA Buffer A to a microcentrifuge and centrifuge the tube at 14,000 rpm for 2 minutes to separate the phases.

12. Transfer the 200 µl aqueous (top) fraction to a fresh 1.5 ml microfuge tube. After the 2 minute centrifugation step, remove the 1.5 ml tube from the microcentrifuge, open the cap and remove the 200 µl DNA sample using a 200 µl tip fitted with barrier plug. Pipette the top layer slowly and carefully to avoid transferring the bottom layer or interface. Transfer the 200 µl aqueous DNA sample to a fresh 1.5 ml microfuge tube.

13. Add 600 µl of DNA Buffer C and vortex for 10 sec. To the 200 µl aqueous DNA sample, add 600 µl of DNA Buffer C using a 1.0 ml pipette tip, close the cap of the 1.5 ml microfuge tube and vortex vigorously for 10 seconds to mix the DNA sample and DNA Buffer C. The total sample volume at this step is 800 µl.

14. Transfer the 800 µl DNA sample to a spin column seated in a microfuge wash tube. Place a spin column into a 2 ml microfuge wash tube and add the 800 µl DNA sample directly onto the spin column membrane making certain to avoid contact between the membrane and the 1.0 ml pipette tip.

15. Centrifuge the spin column assembly at 1,000 rpm for 10 sec and discard the eluent. Centrifuge the spin column assembly containing the 800 µl DNA sample at 1,000 rpm or 1,250 x g for 10 seconds to trap the DNA molecules on the spin column membrane. The buffer components and contaminants will pass through the spin column membrane into the 1.5 ml microfuge wash tube. After the 10 second centrifugation step, remove the spin column assembly from the microcentrifuge and discard the 800 µl eluent in the 1.5 ml microfuge wash tube containing the buffer components and contaminants. At this step, the DNA molecules adhere to the spin column membrane.

16. Add 125 µl of DNA Buffer D to the spin column. After decanting the 800 µl flow-through, place the spin column containing the DNA molecules into the 1.5 ml microfuge wash tube and add 125 µl of DNA Buffer D using a 200 µl pipette tip. This step removes residual contaminants from the spin column membrane.

17. Centrifuge the spin column assembly at 1,000 rpm for 10 sec and discard the eluent. After adding 125 µl of Wash Buffer D to the spin column, centrifuge the spin column assembly at 1,000 rpm for 10 seconds to wash the spin column membrane and discard the flow through. Place the spin column back into the 1.5 ml microfuge wash tube and place the spin column assembly in the microcentrifuge.

18. Repeat wash steps 16 and 17 twice more. After the first 125 µl wash step with Wash Buffer D, repeat this step (steps 16 and 17) two more times to thoroughly wash the spin column membrane. The three wash steps ensure a highly purified DNA preparation upon elution.

19. Centrifuge the spin column assembly at 1,000 rpm for 60 sec to dry the spin column. After the three wash steps, insert the spin column assembly into the microcentrifuge and centrifuge at 1,000 rpm for 60 seconds to dry the spin column completely. This step ensures efficient elution of the DNA sample from the spin column membrane.

20. Add 50 µl of DNA Buffer E directly onto the spin column membrane. After the 60 second centrifugation step, remove the spin column assembly from the microcentrifuge and discard the 1.5 ml microfuge wash tube. Place the spin column containing the highly purified DNA molecules into a fresh 1.5 ml microfuge tube and add 50 µl of DNA Buffer E directly onto the spin column membrane using a 200 µl pipette tip.

21. Incubate the spin column for 5 min to resolubilize the DNA. The 50 µl volume of DNA Buffer E should be allowed to incubate for 5 minutes on the spin column membrane to resolubilize the DNA molecules. Make sure that DNA Buffer E covers the entire membrane during this step.

22. Centrifuge the spin column assembly at 1,000 rpm for 60 sec to elute the DNA. After the 5 minute incubation step, centrifuge the spin column assembly at 1,000 rpm for 60 seconds to elute the DNA sample from the membrane. The 50 µl flow through will collect in the bottom of the 1.5 ml microfuge tube. Discard the spin column and retain the 1.5 ml microfuge tube that contains the purified DNA sample.

23. Dry the DNA sample by vacuum centrifugation for downstream use. After the DNA elution step, place the 1.5 ml microfuge tube containing the 50 µl purified DNA sample in a vacuum centrifuge (e.g. SpeedVac®) and centrifuge the sample for 30 minutes to dry the DNA pellet completely. A 6 mm sample disc should provide approximately 0.5 µg of total genomic DNA.

Serum Isolation (Short Protocol)

Note: Please wear nitrile gloves and safety glasses at all times during this procedure.

1. Excise 6 mm disc from serum portion of blood card.

2. Wet 6 mm disc with 10 µl of 1X Serum Elution Buffer.

3. Place wetted disc in Serum Spin Column assembly.

4. Re-hydrate disc for 30 min at room temperature.

5. Spin assembly for 1 min at 14,000 x g to elute serum.

6. Add 10 µl of 1X Serum Elution Buffer to disc.

7. Spin assembly for 1 min at 14,000 x g to elute residual serum.

8. Mix and react 20 µl serum sample with protein microarray.

Serum Isolation (Complete Protocol)

Note: Please wear nitrile gloves and safety glasses at all times during this procedure.

1. Excise 6 mm disc from serum portion of blood card. Obtain Blood Card (Cat. ABC) containing a chromatographed 5-drop whole blood sample into the red portion (left) and serum protein (right). Use an Arrayit Blood Card Sample Disc Punch (Cat. ASDP) to excise a 6 millimeter (6 mm) disc from the serum portion of the card. The 6 mm will contains antibodies, antigens and other serum proteins of interest.

2. Wet 6 mm disc with 10 µl of 1X Serum Elution Buffer. Using a 20 µl pipette fitted with a 20 µl barrier tip, wet the 6 mm serum disc with 10 µl of 1X Serum Elution Buffer. The filter disc should be held and handled using fine-tipped forceps.

3. Place wetted disc in Serum Spin Column assembly. Place a Serum Spin Column inside a 1.5 ml Serum Collection Tube to form a Serum Spin Column assembly. Place the 6 mm filter disc wetted with 10 µl of 1X Serum Elution Buffer inside the Serum Spin Column portion of the Serum Spin Column assembly.

4. Re-hydrate disc for 30 min at room temperature. The wetted 6 mm serum disc inside the Serum Spin Column assembly should be incubated for 30 min at room temperature to allow re-hydration of the serum proteins. The assembly should be held in a microfuge rack or microfuge rotor in an upright position during 30 min incubation.

5. Spin assembly for 1 min at 14,000 x g to elute serum. The Serum Spin Column assembly containing the 6 mm disc wetted with 10 µl of 1X Serum Elution Buffer should be centrifuged for 1 min at 14,000 x g to elute the serum proteins off the disc and into the 1.5 ml Serum Collection Tube. The volume of the eluted and collected sample should be approximately 10 µl.

6. Add 10 µl of 1X Serum Elution Buffer to disc. Following the first elution, a small percentage of serum proteins remain bound to the 6 mm disc. To remove the remaining bound proteins, add a second 10 µl aliquot of 1X Serum Elution Buffer to the 6 mm disc. The two 10 µl aliquots of 1X Serum Elution Buffer will afford a 20 µl final sample volume. Certain protein microarray assays may require an assay volume greater than 20 µl. In this case, simply increase the volume of the second elution to a volume that is 10 µl less than the assay volume. To obtain a 100 µl final assay volume, for example, the first and second elution volumes should be 10 µl and 90 µl, respectively.

7. Spin assembly for 1 min at 14,000 x g to elute residual serum. After the second 10 µl volume of 1X Serum Elution Buffer is added to the 6 mm serum disc, spin the Serum Spin Column assembly for 1 min at 14,000 x g in a microfuge to elute the serum proteins off the disc and into the 1.5 ml Serum Collection Tube. If two 10 µl volumes of 1X Serum Elution Buffer are used for the elutions, the final volume of eluted serum proteins should be approximately 20 µl. The serum protein elution volume will be higher for assays that use a larger second elution volume.

8. Mix and react 20 µl serum sample with protein microarray. The 1.5 ml Serum Collection Tube will contain the eluted serum sample from the two elutions. For the standard elution protocol that uses 2 x 10 µl of 1X Serum Elution Buffer, the eluted sample will be approximately 20 µl. Mix the eluted sample gently for 5 sec using a rotary vortex to ensure that the sample is completely homogeneous before applying the eluted serum sample to the protein microarray.

Technical Assistance

Please contact us if you have any comments, suggestions, or if you need technical assistance. We can be reached by electronic mail arrayit@arrayit.com from Monday-Friday 8:00 AM-8 PM PST. Please remember that we want to hear about your successes!

Troubleshooting Tips

• The lancet is not creating sufficient blood flow - make sure to press down firmly on the lancet sampling platform during the sampling process

 

• Blood flow stops before 5 droplets are collected - make sure to wash your hands with hot soapy water prior to sampling and stand up during the collection process

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Scientific Publications

Click here and here for Arrayit blood card scientific publications.

Ordering Information

Warranty

Arrayit life sciences products are sold for research purposes only. Arrayit brand products have been scientifically developed and are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All Arrayit brand products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any performance issues should be reported to Arrayit immediately. Arrayit’s liability is limited to the replacement of the product, or a full refund. Any misuse of this product is the full responsibility of the user, and Arrayit makes no warranty or guarantee under such circumstances. Pricing may vary up to 30% due to costs associated with distribution, import taxes, duties, customs clearance and shipping.