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BlockIt Microarray Blocking Buffer, 250 ml of 1X solution


Price: $364.00
Item Number: BKT

Quantity Discounts - Order a quantity in the range below to receive the discount

QuantityAmount
5 to 9$345.80
10 to 19$327.60
20 to 100$309.40
Arrayit BlockIt Microarray Blocking Buffer is formulated for blocking the portions of microarrays that are still reactive after printing to eliminate fluorescent background following the incubation or hybridization reactions of protein and DNA microarrays. Ultra-pure reagents used for buffer formulatiom are purified by sterile filtration and provided as ready-to-use 1X solution, BKT is recommended for SuperEpoxy slides and is compatible with other DNA and protein microarray slides including SuperNitro. Easy coverslip or tray-based blocking procedures efficiently blocks hundreds of microarrays. Ships overnight on wet ice as 250 ml of 1X buffer.

Reagents - Microarray Buffers - Blocking Solutions - BlockIt™ and BlockIt™ HT Blocking Buffers for DNA and Protein Microarrays

blocking-buffer
Arrayit has developed and manufactured an advanced microarray blocking solution designed to inactivate reactive groups remaining post-printing on SuperEpoxy 3SuperAmine 3SuperAldehyde 3 and other glass microarray substrate slide surfaces. BlockIt™ Blocking Solution greatly reduces background noise while maintaining full signal intensities for a wide spectrum of DNA and protein microarray applications. 250 ml of 1X buffer.

Table of Contents

  • Introduction
  • Quality Control
  • Product Description
  • Technical Assistance
  • Short Protocol
  • Complete Protocol
  • Short Protocol DNA
  • Complete Protocol DNA
  • Troubleshooting Tips
  • Recommended Requirements
  • Ordering Information
  • Warranty

Introduction
Congratulations on taking a big step towards improving the economies of scale, quality and speed of your genomics research. This booklet contains a complete set of protocols outlining the steps and principles needed to use the Arrayit brand BlockIt blocking buffer for microarray surface chemistries. This product is design for use prior to incubation and hybridization reactions involving protein and DNA microarrays.

Quality Control
Arrayit assures the performance of these products, the finest scientific research went into their development. Store at 4°C, the product has a 1-year shelf life when handled and stored properly.

Product Description
Arrayit brand BlockIt™ buffer is designed for blocking the portions of microarrays that are still reactive after printing to eliminate fluorescent background following the incubation or hybridization reaction of protein and DNA microarrays. Users will appreciate the following features:

  • Ultra-pure reagents used for buffer formulation
  • Purified by sterile filtration
  • Provided as 1X ready to use concentrate
  • Recommended for use on all types of protein or DNA microarray glass microarray surface chemistries
  • Easy cover slip blocking procedure for minimal reagent consumption
  • 250 ml volume provided efficiently blocks thousands of microarrays
  • Will not cross react with proteins and DNA

Technical Assistance
Please contact us if you have any comments, suggestions, or if you need technical assistance. By electronic mail: arrayit@arrayit.com (under the subject heading, please type Arrayit technical assistance). By email: arrayit@arrayit.com, Monday–Friday PST 8:00am - 8:00pm. Please remember that we want to hear about your successes!

Short Protocol for Protein Microarrays
Wash microarray and then incubate microarray in 1X BlockIt buffer under a coverslip for 1 hour prior to protein binding or hybridization reactions.

Complete Protocol for Protein Microarrays
BlockIt will couple to un-reacted binding groups on microarray surfaces and prevent background fluorescence. Once the printing process is complete, and samples are coupled to the surface, block a microarray surface by using a 1-24 hour incubation at room temperature in 1X BlockIt buffer. Perform this reaction under a coverslip using 4 ul of BlockIt for every cm2 of coverslip area. A Hybridization Cassette should be used to keep the blocking reaction from drying out. After blocking, wash the microarrays to remove excess buffer. Wash 2 times for 15 minutes each in a High Throughput Wash Station in 1X PBST to remove unbound protein molecules and buffer components from the microarray. Protein and DNA binding to the SuperEpoxy 3 surface is extremely stable and the microarrays can be washed, blocked and reacted without sufficient loss of coupled protein.

NOTE: BlockIt can be used as a reaction buffer for all protein microarray applications.

microarray-blocking-buffer
Figure 1. BlockIt™ background fluorescence reduction on SuperAmine (left panel) and SuperEpoxy (right panel) microarray glass substrate slides. Blocking reactions were performed at room temperature for 16 hours. 10 µl of BlockIt™ was used under 22 x 22 mm coverslips. The substrate slides were washed in 1x PBS in a High Throughput Wash Station for 10 minutes at medium speed on a stir plate. The substrate was spun in a Microarray High-Speed Centrifuge until dry. A total of 30 µl of 0.6% Cy3-labeled IgG was reacted across the substrate slides under 20 x 60 mm coverslips. The IgG was suspended in a 1% BSA in PBS solution. The solution was allowed to react for 4 hours at room temperature protected from light. The substrate slides were washed in 1x PBS in a High Throughput Wash Station for 10 minutes and spun dry. The substrate slides were scanned using a PerkinElmer ScanArray Express set at 70% PMT and 90% laser power at a 10 µm resolution. The signal intensities (see Table) were averaged across a circular area with an 8,575 µm diameter. The upper panel shows the scanned images and the table records the signal intensities. BlockIt™ blocking solution reduces background noise by 2-10 fold depending on the surface.

Glass Substrate Slide

- BlockIt™ (fluorescent counts)

+ BlockIt™ (fluorescent counts)

SuperAmine Microarray Substrates

5,586

2,924

SuperEpoxy Microarray Substrates

2,079

698

Short Protocol for DNA Microarrays
Incubate microarray in 1X BlockIt™ buffer under a coverslip or in a microarray reaction tray for 15-60 min prior to hybridization.

Complete Protocol for DNA Microarrays
BlockIt will couple to the un-reacted groups on the surfaces and prevent background fluorescence. Once the printing process is complete, and samples are bound to the surface use a 1-24 hour incubation at room temperature of 1X BlockIt buffer. Perform this reaction under a cover slip using 4 ul of BlockIt for every cm2 of coverslip area. A Hybridization Cassette can be used to keep the blocking reaction from drying out. After blocking wash the printed microarrays in a High Throughput Wash Station using the protocol recommended by the manufacturer. DNA binding to the SuperAmine 3SuperAldehyde 3 and SuperEpoxy 3 surfaces is extremely stable and the microarrays can be washed, blocked and reacted without significant loss of coupled DNA. After blocking, wash and process the microarrays according to the manufacturers recommendation to remove excess buffer and unbound sample.

microarray-blocking-solution
Figure 2.
 Shown is a microarray blocking reaction using BlockIt™ Microarray Blocking Buffer.  Three DNA microarrays are incubated overnight with gentle agitation in a square culture dish containing 30 ml of 1X BlockIt™ to inactivate the microarray surface and reduce fluorescent background during the hybridization reaction. BlockIt™ molecules bind covalently to un-reacted groups on the microarray surface, preventing non-specific binding of probe molecules during hybridization. Unlike other blocking solutions that contain cloudy additives and impure reagents, BlockIt™ is perfectly clear solution of highly purified and soluble components that provides a vastly superior blocking function.  BlockIt™ is an all-purpose blocking solution for DNA, protein, and other types of microarrays.

Troubleshooting Tips
If microarray shows high background:

  • Confirm that the intrinsic background of the microarray is low to start with.
  • Be sure to not let any sample dry on the microarray surface during the binding reaction.
  • Make sure to purify away unincorporated dyes in the labeling reaction.
  • Take care to block for the recommended time (1 hour).
  • Pay attention to the proper handling and shelf life of the buffer
    (1 year shelf life, Store at 4 C).
  • Do not skip washing the microarray after the blocking step.

Recommended Products:

Reagents - Microarray Buffers - Blocking Solutions - BlockIt™ Plus Microarray Blocking Buffer for Enhanced DNA and Protein Glass Substrate Slide Microarrays

Arrayit-BlockIt.2
BlockIt™ Plus microarray blocking solution designed to inactivate reactive groups remaining post-printing on SuperEpoxy 3SuperAmine 3SuperAldehyde 3SuperNitroSuperPVDF and SuperNylon and other microarray substrate slide surfaces. BlockIt™ Plus Blocking Solution greatly reduces background noise while maintaining full signal intensities for a wide spectrum of microarray applications. 250 ml of 1X buffer.

Table of Contents

  • Introduction
  • Quality Control
  • Product Description
  • Technical Assistance
  • Short Protocol
  • Complete Protocol
  • Short Protocol DNA
  • Complete Protocol DNA
  • Troubleshooting Tips
  • Recommended Requirements
  • Ordering Information
  • Warranty

Introduction
Congratulations on taking a big step towards improving the economies of scale, quality and speed of your microarray research. This booklet contains a complete set of protocols outlining the steps and principles needed to use BlockIt™ Plus blocking buffer for microarray surface chemistries. This product is design for use prior to incubation and hybridization reactions involving protein and DNA microarrays.

Quality Control
Arrayit assures the performance of these products, the finest scientific research went into their development. Store at 4°C, the product has a 1-year shelf life when handled and stored properly.

Product Description
Arrayit BlockIt™ Plus blocking buffer is designed for blocking the portions of microarrays that are still reactive after printing to eliminate fluorescent background following the incubation or hybridization reaction of protein and DNA microarrays. Users will appreciate the following features:

  • Often works when regular BlockIt™ is not sufficient
  • Ultra-pure reagents used for buffer formulation
  • Provided as 1X ready to use concentrate
  • Recommended for use on all types of protein or DNA microarray glass microarray surface chemistries
  • Easy cover slip or petri dish blocking procedure for minimal reagent consumption
  • 250 ml volume provided can efficiently block thousands of microarrays
  • Will not cross react with proteins and DNA

Technical Assistance
Please contact us if you have any comments, suggestions, or if you need technical assistance. By electronic mail: arrayit@arrayit.com (under the subject heading, please type Arrayit technical assistance). By email: arrayit@arrayit.com, Monday–Friday PST 8:00am - 8:00pm. Please remember that we want to hear about your successes!

Short Protocol for Protein Microarrays
Wash microarray and then incubate microarray in 1X BlockIt™ Plus buffer under a coverslip for 1 hour prior to protein binding or hybridization reactions.

Complete Protocol for Protein Microarrays
BlockIt will couple to un-reacted binding groups on microarray surfaces and prevent background fluorescence. Once the printing process is complete, and samples are coupled to the surface, block a microarray surface by using a 1-24 hour incubation at room temperature to 4 degress celcius in 1X BlockIt™ Plus buffer. Perform this reaction under a coverslip using 4 ul of BlockIt for every cm2 of coverslip area, also compatible with LifterSlips. A Hybridization Cassette can be used to keep the blocking reaction from drying out. After blocking, wash the microarrays to remove excess buffer. Wash 2 times for 15 minutes each in a High Throughput Wash Station in Protein Microarray Wash Buffer to remove unbound protein molecules and buffer components from the microarray. Protein and DNA binding to the SuperEpoxy 3SuperNitro and SuperPVDF surfaces is extremely stable and the microarrays can be washed, blocked and reacted without sufficient loss of coupled protein.

NOTE: BlockIt™ Plus is not recommended to be used as a reaction buffer for protein microarray applications.

Short Protocol for DNA Microarrays
Incubate microarray in 1X BlockIt™ Plus buffer under a coverslip or in a microarray reaction tray for 15-60 min prior to hybridization.

Complete Protocol for DNA Microarrays
BlockIt™ Plus will couple to the un-reacted groups on the surfaces and prevent background fluorescence. Once the printing process is complete, and samples are bound to the surface use a 1-24 hour incubation at room temperature of 1X BlockIt Plus buffer. Perform this reaction under a cover slip using 4 ul of BlockIt for every cm2 of coverslip area. A Hybridization Cassette can be used to keep the blocking reaction from drying out. After blocking wash the printed microarrays in a High Throughput Wash Station using the protocol recommended by the manufacturer. For best results use Arrayit DNA Microarray Wash Buffers. DNA binding to the SuperAmine 3SuperAldehyde 3 and SuperEpoxy 3 surface is extremely stable and the microarrays can be washed, blocked and reacted without significant loss of coupled DNA. After blocking, wash and process the microarrays according to the manufacturers recommendation to remove excess buffer and unbound sample.

blocking-microarrays
Figure 1.
 Shown is a microarray blocking reaction using BlockIt™ Plus Microarray Blocking Buffer.  Three DNA microarrays can be incubated overnight with gentle agitation in a square culture dish containing 30 ml of 1X BlockIt™ to inactivate the microarray surface and reduce fluorescent background during the hybridization reaction. BlockIt™ Plus molecules bind covalently to un-reacted groups on the microarray surface, preventing non-specific binding of probe molecules during hybridization. BlockIt™ Plus is not a clear solution, but contains highly purified and soluble components that provide a highly efficient blocking function.  BlockIt™ Plus is an all-purpose blocking solution for DNA, protein, and other types of microarrays and is used when blocking with BlockIt™ shows to be inefficient.

Troubleshooting Tips
If microarray shows high background:

  • Confirm that the intrinsic background of the microarray is low to start with.
  • Be sure to not let any sample dry on the microarray surface during the binding reaction.
  • Make sure to purify away unincorporated dyes and other conjugates such as biotin, AP and HRP in the labeling reaction.
  • Take care to block for the recommended time (at least 1 hour).
  • Pay attention to the proper handling and shelf life of the buffer
    (1 year shelf life, Store at 4 C).
  • Do not skip washing the microarray after the blocking step.

Recommended Products:

Reagents - Microarray Buffers - Blocking Solutions - SuperProtein Blocking Buffer to Enhance Signal Strength and Reduce Non-Specific Binding on Nitrocellulose Microarrays

membrane-microarray-blocking
Designed to block SuperProteinSuperPVDFSuperNitro membrane based microarray surface chemistry prior to protein binding reactions for ultra low colorimetric background. For glass based protein microarrays use BlockIt.  For assays sensitive to low amounts of detergent use SuperProtein Blocking Buffer.

Table of Contents

  • Introduction
  • Quality Control
  • Product Description
  • Technical Assistance
  • Short Protocol
  • Complete Protocol
  • Troubleshooting Tips
  • Recommended Requirements
  • Ordering Information
  • Warranty

Introduction
Congratulations on taking a big step towards improving the economies of scale, quality and speed of your proteomics research. This booklet contains a complete set of protocols outlining the steps and principles needed to use the Arrayit brand blocking buffer for  membrane-based surface chemistry. This product is design for use prior to incubation and hybridization reactions involving protein and DNA microarrays.

Quality Control
Arrayit assures the performance of these products, the finest scientific research went into their development.  Store at 4 Degrees Celsius, the product has a 1-year shelf life when handled and stored properly.

Product Description
The buffer is designed for blocking the portions of microarrays that can bind proteins after printing to eliminate background following the incubation or hybridization reaction of protein microarrays. Users will appreciate the following features:

  • Ultra-pure reagents used for buffer formulation
  • Purified by sterile filtration
  • Provided as 1X ready to use concentrate
  • Recommended for use on SuperProtein Substrates, but compatible with other protein membrane based microarray surface chemistries
  • Easy coverslip blocking procedure
  • 250 ml volume provided efficiently blocks thousands of microarrays
  • Will not react with proteins and DNA

Technical Assistance
Please contact us if you have any comments, suggestions, or if you need technical assistance. By electronic mail: arrayit@arrayit.com (under the subject heading, please type Arrayit technical assistance). By email: arrayit@arrayit.com, Monday–Friday PST 8:00am - 8:00pm. Please remember that we want to hear about your successes!

Short Protocol for Protein Microarrays
Wash microarray with PBS and then incubate microarray in 1X blocking buffer under a coverslip for 1 hour prior to protein binding reactions.

Complete Protocol for Protein Microarrays
Protein binding to our membrane surfaces is stable and the microarrays can be washed, blocked and reacted without sufficient loss of coupled protein. Block the printed microarray surface using a 1-hour incubation at room temperature in 1X SuperProtein Blocking buffer. Perform this reaction under a coverslip using 4 ul of buffer for every cm2 of coverslip area. Once the printing process is complete, wash the printed microarrays 2 times for 15 minutes each in a High Throughput Wash Station in 1X PBS to remove unbound protein molecules and buffer components from the SuperProtein Substrates.A Hybridization Cassette can be used to keep the blocking reaction from drying out. SuperProtein blocking buffer will couple to un-reacted binding sites of the surface and prevent unspecific binding and background. After blocking, wash the microarrays to remove excess buffer. Washing three times for 1 min each at room temperature with 1X PBS in a High Throughput Wash Station. DO NOT USE DETERGENT BASED buffers on the SuperProtein nor SuperPVDF, low concentrations are compatible with SuperNitro.

Troubleshooting Tips
If microarray shows high background:

  • Confirm that the intrinsic background of the microarray is low to start with.
  • Be sure to not let any sample dry on the microarray surface during the binding reaction.
  • Make sure to purify away unincorporated components of any labeling reactions.
  • Take care to block for the recommended time (1 hour).
  • Pay attention to the proper handling and shelf life of the buffer (1 year shelf life, Store at 4 C).
  • Do not skip washing the microarray after the blocking step.
  • If colorimetric labeling was used, be sure to optimize development times

Recommended Products

Ordering Information

Product

Description

Catalog ID

BlockIt™ Blocking Solution

Arrayit BlockIt™ Blocking Solution is an advanced microarray blocking solution designed to inactivate reactive groups remaining post-printing on SuperEpoxy, SuperAmine, SuperAldehyde and other glass microarray substrate slide surfaces. BlockIt™ Blocking Solution greatly reduces background noise while maintaining full signal intensities for a wide spectrum of DNA and protein microarray applications. Ultra-high purity blocking buffer for coverslip and solution-based blocking of protein and DNA microarrays, 250 ml of 1X buffer, ships on cold packs.

BKT

BlockIt™ HT Blocking Solution

Arrayit BlockIt™ HT Blocking Solution is an advanced microarray blocking solution designed to inactivate reactive groups remaining post-printing on SuperEpoxy, SuperAmine, SuperAldehyde and other glass microarray substrate slide surfaces. BlockIt™ Blocking Solution greatly reduces background noise while maintaining full signal intensities for a wide spectrum of DNA and protein microarray applications. High purity blocking buffer for bulk solution high-throughput (HT) blocking of protein and DNA microarrays for use with the High Throughput Wash Station, 1000 ml of 1X buffer, ships on cold packs.

BKTHT

BlockIt™ Plus Blocking Buffer

Arrayit BlockIt™ Plus Blocking Solution often works when BlockIt™ is shown to be less efficient than required. Greatly reduces background noise while maintaining full signal intensities for a wide spectrum of DNA and protein microarray applications. Ultra-high purity blocking buffer (translucent), for blocking of protein and DNA microarrays, 250 ml of 1X buffer, ships on cold packs.

BKTP

Microarray Blocking Buffer Plus Automation Formulation

Arrayit Microarray BlockIt™ Plus Blocking Solution Automation Formulation should be used in cases where a greatly reduced background is required, while maintaining full signal intensities for a wide spectrum of DNA and protein microarray applications particularly those utilizing workstations, liquid handling robots, multiplex hybridization cassettes and other high-throughput hardware and automation equipment. 1L of 1X buffer arrives pre-mixed and ready to use, ships on cold packs.

BKTPL

SuperProtein Substrate Blocking Buffer

Arrayit SuperProtein Substrate Blocking Buffer is an advanced microarray blocking solution designed to inactivate reactive groups remaining post-printing on our membrane-based microarray surfaces such as nitrocellulose, PVDF and nylon. SuperProtein Blocking Solution greatly reduces background noise while maintaining full signal intensities for a wide spectrum of protein microarray applications. 250 ml of 1X buffer, ships on cold packs.

SPBB

SuperStreptavidin Microarray Blocking Buffer

Arrayit SuperStreptavidin Microarray Blocking Buffer is an advanced protein and small molecule blocking buffer that blocks and inactivates SuperStreptavidin microarray substrate slides prior to staining, greatly reduces background for all applications, 250 ml of 1X buffer solution, ships on cold packs.

SBB

SuperAvidin Blocking Buffer

Arrayit SuperAvidin Blocking Buffer is an advanced protein and small molecule blocking buffer that blocks and inactivates SuperAvidin microarray substrate slides prior to staining, greatly reduces background for all applications, 250 ml of 1X buffer solution, ships on cold packs.

ABB

ChemBlock™ Microarray Chemical Blocking Buffer

Arrayit ChemBlock™ Microarray Blocking Buffer features a proprietary mixture of chemical blocking agents, buffering components and detergents to inactive aldehyde and epoxide reactive groups after microarray printing and before DNA and protein microarray reactions to eliminate background binding observed with traditional protein blocking buffers. ChemBlock™ is highly recommended for serum reactions and other protein microarray applications requiring uniform and extremely low background signals across a large number of blood specimens, 0.2 µm filtered, store at -20°C, 1 liter of 1X solution, ships on cold packs.

CHE

 

Warranty

Arrayit life sciences products are sold for research purposes only. Arrayit brand products have been scientifically developed and are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All Arrayit brand products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any performance issues should be reported to Arrayit immediately. Arrayit’s liability is limited to the replacement of the product, or a full refund. Any misuse of this product is the full responsibility of the user, and Arrayit makes no warranty or guarantee under such circumstances. Pricing may vary up to 30% due to costs associated with distribution, import taxes, duties, customs clearance and shipping.

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