MMH96 Silicone Replacement Gasket

Arrayit MMH96 Silicone Replacement Gasket is for hybridization cassette MMH96 with 96 reaction wells to empower DNA and protein microarrays in a 96-well format. MMH96 Silicone Replacement Gasket for the Arrayit Microplate Microarray Hybridization Cassette MMH96. The silicone replacement gasket creates individual 7.7 x 7.7 mm wells on the glass substrate in an 8 x 12 pattern for 96-well microplate formatted microarrays, 1 silicone replacement gasket per shipment.

Tools - Microarray Hybridization - Microplate Microarray Cassettes and Microplate Glass Substrates for Microarray Sample Multiplexing in the 96-Well Format

Arrayit offers innovative microplate and glass microplate substrate solutions for microplate-formatted microarray experimentation and processing in the standard Society for Biomolecular Sciences (SBS) format. Arrayit microplate microarray hardware including the MMH96 cassette shown above are precision machined from durable alloy and include silicone gaskets for well sealing. Arrayit glass microplate substrates feature amine, aldehyde and epoxy surface chemistry as well as clean substrates for customers wishing to add their own chemistry.

Table of Contents

• Product Description
• Glass Specifications
• Microplate Microarray Cassettes and Substrates
• Technical Assistance
• Troubleshooting Tips
• Scientific Publications
• Ordering Information
• Warranty

Product Description
Arrayit offers premium microarray microplate hybridization cassettes and glass microplate substrates for microarray experimentation and processing in the widely-used SBS microplate format. These cassettes are highly durable and the glass substrates can be used with NanoPrint 2 microarrayers, 946 microarray contact printing technology and microplate printing modules, which are designed for high throughput manufacturing in the 96 well format. This technology combination allows 1,000 DNA or protein samples to be printed in each 96-well sub-array on the glass microplate substrate for high-throughput testing of patient research specimens, therapeutic compound screening and other exciting applications.

Arrayit surface chemistries set the standard for all microarray applications. These links provide detailed information on performance, protocols and applications.

• SuperClean substrate slides are untreated and are delivered ready to use for custom surface chemistry preparation applications. This superior glass is cut to precision dimensions and polished to atomic smoothness (20-25 angstroms over 25 x 76 mm) to provide the most homogenous surface available in industry. SuperClean Microarray Substrate slides have an average smoothness of 2.0 nanometers or 20 angstroms, which corresponds to approximately 10 silicon dioxide bonds. SuperClean Glass Microplates (Cat. PMCG96) provide super clean surface chemistry in the 1 x 77 x 118 mm microplate format.
• SuperAmine chemistry is used to immobilized unmodified long oligos and cDNAs for gene expression microarrays and other applications. SuperAmine Microarray Substrate slides have an average smoothness of 2.0 nanometers or 20 angstroms, which corresponds to approximately 10 silicon dioxide bonds. SuperAmine Glass Microplates (Cat. PMMG96) provide super amine surface chemistry in the 1 x 77 x 118 mm microplate format.
• SuperAldehyde is used to immobilize amino-modified oligos, amino modified cDNA, proteins, cells, lysates, tissues, etc. This surface is specifically useful in immobilizing short oligos for single nucleotide polymorphism (SNP) detection microarrays. SuperAldehyde has been used in many protein microarray applications. SuperAldehyde Microarray Substrate slides have an average smoothness of 2.0 nanometers or 20 angstroms, which corresponds to approximately 10 silicon dioxide bonds. SuperAldehyde Glass Microplates (Cat. PMAG96) provide super aldehyde surface chemistry in the 1 x 77 x 118 mm microplate format.
• SuperEpoxy chemistry is great for protein microarrays. It is also an excellent surface to immobilize oligonucleotides, cells, tissues, and carbohydrates. SuperEpoxy Microarray Substrate slides have an average smoothness of 2.0 nanometers or 20 angstroms, which corresponds to approximately 10 silicon dioxide bonds. SuperEpoxy Glass Microplates (Cat. PMEG96) provide super epoxy surface chemistry in the 1 x 77 x 118 mm microplate format.
• Custom chemistries available, please call for a quote.

Figure 1. Arrayit offers clean, amine, aldehyde and epoxy surface chemistries for glass microplate microarray (1 x 77 x 118 mm) applications. These surface chemistries enable customers to print and attach DNA and protein molecules of interest in the 96-well format, and to process the microarrays with patient research specimens using liquid handling robots and other automation instrumentation for high-throughput applications in a microarray format.

Glass Specifications
Arrayit uses glass specifications specifically chosen for glass microplate microarray applications. Our highly polished glass surfaces provide superior homogeneity. Please see the following specifications:

1. Microplate formatted glass substrates (0.96 x 75 x 118 mm)
2. Smooth to less than 50 angstroms across the 75 x 118 mm substrate
3. Refractive index of 1.52 (400-700 nm)
4. Transmission of >91% (380-700 nm)
5. Thermal strain point of 490°C
6. Harder than standard glass
7. Extended shelf life of surface chemistries

Microplate Microarray Cassettes and Substrates

Figure 2. Arrayit Microplate Microarray Hybridization Cassette MMH96 shown fully-assembled with the cassette top, silicone gasket, glass microplate substrate and cassette base. The MMH96 cassette is assembled and sealed using the four thumb screws located in the cassette corners. The numbers 1-12 across the top and the letters A-H down the top identify each of the 96 wells. The MMH96 cassette has SBS compliant base (bottom) external dimensions of 85 x 126 mm.

Figure 3. Arrayit microplate glass substrate (0.96 x 77 x 118 mm) use a 96-well teflon mask to demarcate each of the ninety-six 7.7 mm x 7.7 mm wells. Arrayit glass microplate substrates are available in clean (Cat. PMCG96), amine (Cat. PMMG96), aldehyde (Cat. PMAG96) and epoxy (Cat. PMEG96) surface chemistries, enabling customers to print 96 microarrays in each of the 96 wells. Arrayit glass microplate substrates are used in conjunction with MMH96 hybridization cassettes, which have SBS compliant base (bottom) external dimensions of 85 x 126 mm.

Figure 4. Arrayit Microplate Microarray Hybridization Cassette MMH96 (Cat. MMH96) base containing a glass microplate substrate (0.96 x 77 x 118 mm). Arrayit glass microplate substrates are available in SuperClean (Cat. PMCG96), SuperAmine (Cat. PMMG96), SuperAldehyde (Cat. PMAG96), and SuperEpoxy (Cat. PMEG96) surface chemistries. The glass microplate substrates fit inside the base (bottom) of the MMH96 cassette, which has SBS compliant base (bottom) external dimensions of 85 x 126 mm.

Figure 5. Arrayit Microplate Microarray Hybridization Cassette MMH96 (Cat. MMH96) fully assembled in an inverted view, showing the water-tight silicone gasket seated against the glass microplate substrate. The silicone gasket seals each of the 96 wells and enables customers to multiplex 96 different patient research specimens in the 96-well cassette. The MMH96 cassette has SBS compliant external base (bottom) dimensions of 85 x 126 mm.

Figure 6. Arrayit Microplate Microarray Hybridization Cassette (Cat. MMH96) from top to bottom: (1) self-sealing septa mat that has sealable ports to allow pipetting and sealing to prevent evaporation, (2) 96-well cassette top with thumb screws that attach to the base, (3) flexible silicone gasket that creates reaction areas in each of the 96 wells, (4) glass microplate substrate (0.96 x 77 x 118 mm) with teflon pattern for unambiguous glass microplate orientation, (5) cassette base with 96-well SBS microplate compliant 85 x 126 mm external dimensions that attaches to the cassette top to complete the 96-well cassette assembly. The microplate format is also available in the form of 4 standard glass substrate slides (1 x 25 x 76 mm) for users requiring a microplate format while maintaining a microarray glass slide format for printing and scanning (see Cat. MMH4X24).

Figure 7. Arrayit Microplate Microarray Hybridization Cassette MMH4X24 (4 Slides) for 96-well microplate formatted microarrays on glass slides (1 x 25 x 76 mm) that includes a self-sealing septa mat with sealable ports to allow pipetting into the cassette and prevent evaporation during use, 96-well cassette top with thumb screws that attach to the base, flexible silicone gasket that creates the 96 reaction areas on the four glass slides in an 4 x 3 x 8 pattern that comprises the 96 wells on the four glass slides, and a cassette base with 96-well SBS microplate compliant 85 x 126 mm external dimensions that attaches to the cassette top to complete the 96-well cassette assembly. Precision machined from durable alloy for extended use in DNA and protein microarray testing applications, MMH4X24 accommodates four 1 x 25 x 76 mm glass substrate slides. Note that the MMH4X24 cassette top is not SBS compliant (it is slightly larger than the 86 x 128 mm SBS dimensions).